GAD65 anticorps (AA 3-96)
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- Antigène Voir toutes GAD65 (GAD2) Anticorps
- GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))
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Épitope
- AA 3-96
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Reactivité
- Rat, Souris
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp GAD65 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Specificité
- Specific for GAD 2 / GAD 65
- Purification
- purified IgG. Azide was added before lyophilization.
- Immunogène
- Purified recombinant mouse GAD 2 (aa 3-96).
- Clone
- 26H1
- Isotype
- IgG3
- Top Product
- Discover our top product GAD2 Anticorps primaire
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- Indications d'application
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WB: 1 : 1000 (AP staining)
ICC: 1 : 500
IHC: 1 : 200 up to 1 : 500 - Commentaires
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WB: This antibody is less sensitive than the rabbit antibody. ELISA: Suitable as capture antibody for sandwich-ELISA with ABIN1742419 as detector antibody (protocol for sandwich-ELISA).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- Buffer
- PBS
- Conseil sur la manipulation
- Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
- Stock
- -20 °C
- Stockage commentaire
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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Vesicular glutamate transporter 1 orchestrates recruitment of other synaptic vesicle cargo proteins during synaptic vesicle recycling." dans: The Journal of biological chemistry, Vol. 290, Issue 37, pp. 22593-601, (2015) (PubMed).
: "The active zone protein family ELKS supports Ca2+ influx at nerve terminals of inhibitory hippocampal neurons." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 37, pp. 12289-303, (2014) (PubMed).
: "Immunocytochemical identification of electroneutral Na⁺-coupled HCO₃⁻ transporters in freshly dissociated mouse medullary raphé neurons." dans: Neuroscience, Vol. 246, pp. 451-67, (2013) (PubMed).
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Vesicular glutamate transporter 1 orchestrates recruitment of other synaptic vesicle cargo proteins during synaptic vesicle recycling." dans: The Journal of biological chemistry, Vol. 290, Issue 37, pp. 22593-601, (2015) (PubMed).
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- Antigène
- GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))
- Autre désignation
- GAD 2 (GAD2 Produits)
- Synonymes
- anticorps GAD65, anticorps gad65, anticorps F12P19.12, anticorps F12P19_12, anticorps GLUTAMATE DECARBOXYLASE 2, anticorps glutamate decarboxylase 2, anticorps zgc:112198, anticorps 6330404F12Rik, anticorps GAD(65), anticorps Gad-2, anticorps glutamate decarboxylase 2, anticorps glutamate decarboxylase 1, anticorps glutamic acid decarboxylase 2, anticorps GAD2, anticorps PTRG_06148, anticorps LOC9324534, anticorps Gad2, anticorps GAD1, anticorps gad2
- Sujet
- Synonyms: GAD 65
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