alpha Tubulin anticorps (AA 419-435)

N° du produit ABIN1742547

Détail du produit anti-alpha Tubulin anticorps

Antigène: alpha Tubulin (TUBA1)

Épitope: AA 419-435

Reactivité: Humain, Souris, Rat, Saccharomyces cerevisiae, Vertebrate, Invertébré

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp alpha Tubulin est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunoprecipitation (IP)

Specificité: Specific for alpha-tubulin (glu- and tyr-alpha-tubulin).

Purification: purified IgG. Azide was added before lyophilization.

Immunogène: Synthetic peptide SEAREDMAALEKDYEEV (aa 419-435 in human alpha-tubulin) coupled to key-hole limpet hemocyanin via an added N -terminal cysteine residue.

Clone: 3A2

Isotype: IgG1

Information d'application

Indications d'application: WB: 1 : 1000 up to 1 : 50000 (AP staining)
ICC: 1 : 500 up to 1 : 1000
IHC-P: 1 : 200

Commentaires:

ELISA: Suitable as capture antibodies for sandwich-ELISA with ABIN1742550 as detector antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Stock: -20 °C

Stockage commentaire: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Références pour anticorps anti-alpha Tubulin (ABIN1742547)

Jadhav, Katina, Kovac, Kazmerova, Novak, Zilka: "Truncated tau deregulates synaptic markers in rat model for human tauopathy." dans: Frontiers in cellular neuroscience, Vol. 9, pp. 24, (2015) (PubMed).

Shinoda, Ahmed, Ramachandran, Bharat, Brockelt, Altas, Dean: "BDNF enhances spontaneous and activity-dependent neurotransmitter release at excitatory terminals but not at inhibitory terminals in hippocampal neurons." dans: Frontiers in synaptic neuroscience, Vol. 6, pp. 27, (2014) (PubMed).

Spangler, Schmitz, Kevenaar, de Graaff, de Wit, Demmers, Toonen, Hoogenraad: "Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission." dans: The Journal of cell biology, Vol. 201, Issue 6, pp. 915-28, (2013) (PubMed).

Détails sur alpha Tubulin

Antigène: alpha Tubulin (TUBA1)

Autre désignation: alpha-Tubulin (TUBA1 Produits)

Synonymes: anticorps Tuba1, anticorps K-ALPHA-1, anticorps Tuba-1, anticorps cb944, anticorps fb22g06, anticorps tuba1, anticorps wu:fb22g06, anticorps Calpha1, anticorps tubulin, alpha 1A, anticorps tubulin alpha 1b, anticorps tubulin, alpha 1b, anticorps tubulin, alpha 1B, anticorps Tuba1a, anticorps TUBA1B, anticorps tuba1b, anticorps Tuba1b

Pathways: Dynamique des Microtubules