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STK35 anticorps (C-Term)

Verified STK35 Reactivité: Humain, Souris, Porc WB, ELISA, IHC, IF, FACS Hôte: Chèvre Polyclonal unconjugated
N° du produit ABIN185178
  • Antigène Voir toutes STK35 Anticorps
    STK35 (Serine/threonine Kinase 35 (STK35))
    Épitope
    • 15
    • 7
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivité
    • 26
    • 17
    • 11
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain, Souris, Porc
    Hôte
    • 41
    Chèvre
    Clonalité
    • 41
    Polyclonal
    Conjugué
    • 12
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp STK35 est non-conjugé
    Application
    • 30
    • 15
    • 13
    • 13
    • 9
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
    Fonction
    Clik1 / STK35
    Séquence
    ELETRMDQVT CAA
     Réactivité croisée
    Boeuf (Vache), Chien, Humain, Souris, Porc, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Classe de qualité
    Verified
    Immunogène
    Peptide with sequence ELETRMDQVTCAA, from the C Terminus of the protein sequence according to NP_543026.2.
    Isotype
    IgG
  • Indications d'application

    Immunohistochemistry: In paraffin embedded Human Testis shows granular cytoplasmic staining of Leydig cells. Recommended concentration: 10 μg/mL.

    Western Blot: Approx 55 kDa band observed in Human Breast cancer and in Mouse and Pig Testis lysates, and approx. 58 kDa in nuclear lysates of cell line HEK293 (calculated MW of 58.1 kDa according to Human NP_543026.2, 59.5 kDa according to Mouse NP_899085.3

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Commentaires

    Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Minimize freezing and thawing.
    Stock
    -20 °C
    Stockage commentaire
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Goyal, Behring, Kumar, Siess: "Identifying and characterizing a novel protein kinase STK35L1 and deciphering its orthologs and close-homologs in vertebrates." dans: PLoS ONE, Vol. 4, Issue 9, pp. e6981, (2009) (PubMed).

  • Antigène
    STK35 (Serine/threonine Kinase 35 (STK35))
    Autre désignation
    STK35 (STK35 Produits)
    Synonymes
    anticorps clik1, anticorps stk35l1, anticorps MGC69346, anticorps im:6905214, anticorps zgc:153287, anticorps MGC115406, anticorps CLIK1, anticorps STK35L1, anticorps 1700054C12Rik, anticorps Stk35l1, anticorps serine/threonine kinase 35, anticorps serine/threonine kinase 35 S homeolog, anticorps stk35, anticorps stk35.S, anticorps STK35, anticorps Stk35
    Sujet
    STK35, Clik1, bA550O8.2, bA550O8.2 CLIK1, serine/threonine kinase 35, CLP-36 interacting kinase, CLIK1, STK35L1, OTTHUMP00000030006, serine threonine kinase 35 long form
    ID gène
    140901, 67333
    NCBI Accession
    NP_543026
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