AKT3 anticorps (AA 119-133)

N° du produit ABIN185314

Détail du produit anti-AKT3 anticorps

Antigène: AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))

Épitope: AA 119-133

Reactivité: Humain, Souris, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp AKT3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Fonction: AKT3

Séquence: CSPTSQIDNI GEEEM

Specificité: This antibody is expected to recognise an epitope corresponding to aa 119-133 of both isoforms of human AKT3 protein. This antibody does not cross-react with human AKT1/2. Reported variants represent identical protein (NP_859029.1, NP_001193658.1).

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Immunogène: Peptide with sequence CSPTSQIDNIGEEEM, from the internal region of the protein sequence according to NP_005456.1, NP_859029.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Prostate and Heart. Recommended concentration: 5 μg/mL.

Western Blot: A 50-55 kDa band observed in lysates of cell lines HepG2 and Jurkat and in Mouse and Rat Brain lysates (calculated MW of 54.0 kDa according to NP_859029.1). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur AKT3

Antigène: AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))

Autre désignation: AKT3 (AKT3 Produits)

Synonymes: anticorps MPPH, anticorps PKB-GAMMA, anticorps PKBG, anticorps PRKBG, anticorps RAC-PK-gamma, anticorps RAC-gamma, anticorps STK-2, anticorps AI851531, anticorps D930002M15Rik, anticorps Nmf350, anticorps Pkbg, anticorps AKT serine/threonine kinase 3, anticorps thymoma viral proto-oncogene 3, anticorps v-akt murine thymoma viral oncogene homolog 3b, anticorps AKT3, anticorps Akt3, anticorps akt3b

Sujet: AKT3, PKBG, PRKBG, STK-2, RAC-gamma, RAC-PK-gamma, DKFZP434N0250, v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma), protein kinase B gamma, serine threonine protein kinase, Akt-3, RAC-gamma serine/threonine protein kinase, PKB-GAMMA

ID gène: 10000

NCBI Accession: NP_005456, NP_859029

Pathways: Signalisation PI3K-Akt, Signalisation RTK, Signalisation TLR, Hepatitis C, VEGF Signaling