p53 anticorps (AA 364-393)

N° du produit ABIN1882106

Détail du produit anti-p53 anticorps

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Épitope: AA 364-393

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp p53 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogène: This p53 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364-393 amino acids from human p53.

Clone: RB2571

Isotype: Ig Fraction

Information d'application

Indications d'application: WB: 1:1000. WB: 1:2000. IHC-P: 1:100

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Date de péremption: 6 months

Références pour anticorps anti-p53 (ABIN1882106)

Chen, Chen: "MDM2-ARF complex regulates p53 sumoylation." dans: Oncogene, Vol. 22, Issue 34, pp. 5348-57, (2003) (PubMed).

Melchior, Hengst: "SUMO-1 and p53." dans: Cell cycle (Georgetown, Tex.), Vol. 1, Issue 4, pp. 245-9, (2002) (PubMed).

Muller, Berger, Lehembre, Seeler, Haupt, Dejean: "c-Jun and p53 activity is modulated by SUMO-1 modification." dans: The Journal of biological chemistry, Vol. 275, Issue 18, pp. 13321-9, (2000) (PubMed).

Gostissa, Hengstermann, Fogal, Sandy, Schwarz, Scheffner, Del Sal: "Activation of p53 by conjugation to the ubiquitin-like protein SUMO-1." dans: The EMBO journal, Vol. 18, Issue 22, pp. 6462-71, (2000) (PubMed).

Rodriguez, Desterro, Lain, Midgley, Lane, Hay: "SUMO-1 modification activates the transcriptional response of p53." dans: The EMBO journal, Vol. 18, Issue 22, pp. 6455-61, (2000) (PubMed).

Détails sur p53

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Autre désignation: p53 (TP53 Produits)

Synonymes: anticorps BCC7, anticorps LFS1, anticorps P53, anticorps TRP53, anticorps p53, anticorps Trp53, anticorps brp53, anticorps drp53, anticorps etID22686.5, anticorps fb40d06, anticorps wu:fb40d06, anticorps zgc:111919, anticorps Tp53, anticorps bbl, anticorps bfy, anticorps bhy, anticorps p44, anticorps tp53, anticorps Xp53, anticorps TP53, anticorps tumor protein p53, anticorps cellular tumor antigen p53, anticorps transformation related protein 53, anticorps tumor protein p53 L homeolog, anticorps TP53, anticorps CpipJ_CPIJ002758, anticorps Tp53, anticorps tp53, anticorps Trp53, anticorps tp53.L

Sujet: Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. P53 is subject to modification by conjugation of SUMO-1. A p53 mutant deficient for MDM2 binding is poorly sumoylated in vivo compared to wild-type p53. Overexpression of MDM2 increases the level of p53 sumoylation, which is further stimulated by expression of ARF. These results show that p53 sumoylation is regulated by MDM2- and ARF-mediated nucleolar targeting.

Poids moléculaire: 43653

NCBI Accession: NP_000537, NP_001119584, NP_001119585, NP_001119586, NP_001119587, NP_001119588, NP_001119589, NP_001119590, NP_001263624, NP_001263625, NP_001263626, NP_001263627, NP_001263628, NP_00126

UniProt: P04637

Pathways: Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, L'effet Warburg