Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Homologie
M, Rat
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogène
This PIAS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-126 amino acids from the N-terminal region of human PIAS3.
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Date de péremption
6 months
Spoden, Morandell, Ehehalt, Fiedler, Jansen-Dürr, Hermann, Zwerschke: "The SUMO-E3 ligase PIAS3 targets pyruvate kinase M2." dans: Journal of cellular biochemistry, Vol. 107, Issue 2, pp. 293-302, (2009) (PubMed).
Brantley, Nabors, Gillespie, Choi, Palmer, Harrison, Roarty, Benveniste: "Loss of protein inhibitors of activated STAT-3 expression in glioblastoma multiforme tumors: implications for STAT-3 activation and gene expression." dans: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 15, pp. 4694-704, (2008) (PubMed).
Iwasaki, Hailemariam, Tsuji: "PIAS3 interacts with ATF1 and regulates the human ferritin H gene through an antioxidant-responsive element." dans: The Journal of biological chemistry, Vol. 282, Issue 31, pp. 22335-43, (2007) (PubMed).
Nojiri, Joh, Miura, Sakata, Nomura, Nakao, Sobue, Ohara, Asai, Ito: "ATBF1 enhances the suppression of STAT3 signaling by interaction with PIAS3." dans: Biochemical and biophysical research communications, Vol. 314, Issue 1, pp. 97-103, (2004) (PubMed).
Long, Wang, Matsuura, He, Liu: "Activation of Smad transcriptional activity by protein inhibitor of activated STAT3 (PIAS3)." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 1, pp. 99-104, (2004) (PubMed).
Cheng, Zhang, Zhou, Marasco: "Down-regulation of SHP1 and up-regulation of negative regulators of JAK/STAT signaling in HTLV-1 transformed cell lines and freshly transformed human peripheral blood CD4+ T-cells." dans: Leukemia research, Vol. 28, Issue 1, pp. 71-82, (2003) (PubMed).
Yamamoto, Sato, Sekine, Yumioka, Imoto, Junicho, Fuse, Matsuda: "Molecular interactions between STAT3 and protein inhibitor of activated STAT3, and androgen receptor." dans: Biochemical and biophysical research communications, Vol. 306, Issue 2, pp. 610-5, (2003) (PubMed).
Ueki, Seki, Yano, Saito, Masuho, Muramatsu: "Isolation and chromosomal assignment of a human gene encoding protein inhibitor of activated STAT3 (PIAS3)." dans: Journal of human genetics, Vol. 44, Issue 3, pp. 193-6, (1999) (PubMed).
Antigène
PIAS3
(Protein Inhibitor of Activated STAT, 3 (PIAS3))
PIAS3 is a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor, catalyzing the covalent attachment of a SUMO protein to specific target substrates. PIAS3 plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context.