LCK anticorps

N° du produit ABIN1882258

Détail du produit anti-LCK anticorps

Antigène: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp LCK est non-conjugé

Application: Western Blotting (WB)

Purification: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.

Immunogène: Purified His-tagged LCK protein was used to produced this monoclonal antibody.

Clone: 845CT3-5-2

Isotype: IgG1

Information d'application

Indications d'application: WB: 1:5000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Mouse monoclonal antibody supplied in crude ascites with 0.09 % (W/V) sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Date de péremption: 6 months

Références pour anticorps anti-LCK (ABIN1882258)

Yamaguchi, Hendrickson: "Structural basis for activation of human lymphocyte kinase Lck upon tyrosine phosphorylation." dans: Nature, Vol. 384, Issue 6608, pp. 484-9, (1997) (PubMed).

Wright, Sefton, Kamps: "Oncogenic activation of the Lck protein accompanies translocation of the LCK gene in the human HSB2 T-cell leukemia." dans: Molecular and cellular biology, Vol. 14, Issue 4, pp. 2429-37, (1994) (PubMed).

Rouer, Van Huynh, Lavareda de Souza, Lang, Fischer, Benarous: "Structure of the human lck gene: differences in genomic organisation within src-related genes affect only N-terminal exons." dans: Gene, Vol. 84, Issue 1, pp. 105-13, (1990) (PubMed).

Détails sur LCK

Antigène: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Autre désignation: LCK (LCK Produits)

Synonymes: anticorps zgc:136695, anticorps LCK, anticorps Hck-3, anticorps Lsk, anticorps Lskt, anticorps p56, anticorps p56Lck, anticorps LSK, anticorps YT16, anticorps p56lck, anticorps pp58lck, anticorps P56LCK, anticorps tkl, anticorps Lck1, anticorps Lcktkr, anticorps LCK proto-oncogene, Src family tyrosine kinase, anticorps lymphocyte protein tyrosine kinase, anticorps lck, anticorps LCK, anticorps Lck

Sujet: Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP.

Poids moléculaire: 58001

NCBI Accession: NP_001036236, NP_005347

UniProt: P06239

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling