FOXP3 anticorps (Forkhead Box P3)

Details for Product anti-FOXP3 Antibody No. ABIN188539
Antigène
  • foxp3
  • FOXP3
  • AIID
  • DIETER
  • IPEX
  • PIDX
  • XPID
  • JM2
  • scurfin
  • sf
  • RGD1562112
  • forkhead box P3
  • forkhead box P3a
  • forkhead box P3 L homeolog
  • FOXP3
  • foxp3a
  • foxp3
  • foxp3.L
  • Foxp3
Reactivité
Humain, Souris, Rat (Rattus)
317
209
68
41
11
7
4
3
3
2
2
1
1
1
Hôte
Lapin
201
146
38
26
3
Clonalité
Polyclonal
Conjugué
Cet anticorp FOXP3 est non-conjugé
27
25
21
19
14
12
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
260
165
152
99
79
28
27
23
22
19
16
8
6
5
3
3
Options
Immunogène A peptide corresponding to amino acids 43-100 of mouse FOXP3. [Swiss-Prot# Q99JB6]
Purification Epitope affinity purified
Plasmids, Primers & others Plasmids, Primers & others FOXP3 products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation FoxP3 (FOXP3 Antibody Extrait)
Sujet Gene Symbol: FOXP3
ID gène 50943
UniProt Q99JB6
Pathways Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
Indications d'application Western Blot 1:2000, Flow Cytometry 1:8000, Immunohistochemistry 1:400, Immunocytochemistry/Immunofluorescence 1:500, Immunoprecipitation, Immunohistochemistry-Paraffin 1:400This FOXP3 antibody can be used for Immunoprecipitation, Immunohistochemistry paraffin embeded sections and Western blot analysis, where a band is seen ~48 kDa in FOXP3 transfected and CD25/CD4 positive regulatory T cell lysates. In IP transfected FOXP3-HA tagged proteins were used. In IHC-P, staining was observed in the nuclei of mouse spleen cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer ( pH 6.0) is recommended.
Commentaires

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocole Immunohistochemistry-Paraffin Embedded Sections NB100-39002 Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes.Staining:
1. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 degrees C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Avoid freeze-thaw cycles
Stock -80 °C,-20 °C
Stockage commentaire Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Images (Fournisseur)
Western Blotting (WB) image for anti-Forkhead Box P3 (FOXP3) antibody (ABIN188539) Western Blot: FOXP3 Antibody [ABIN1885392] - Detection of immunoprecipitated FOXP3 fr...
Immunohistochemistry (IHC) image for anti-Forkhead Box P3 (FOXP3) antibody (ABIN188539) Immunohistochemistry: FOXP3 Antibody [ABIN1885392] - FOXP3 antibody was tested in mou...
Flow Cytometry (FACS) image for anti-Forkhead Box P3 (FOXP3) antibody (ABIN188539) Flow Cytometry: FOXP3 Antibody [ABIN1885392] - Transfectants from A were permeabilize...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Forkhead Box P3 (FOXP3) antibody (ABIN188539) Immunohistochemistry-Paraffin: FoxP3 Antibody [ABIN1885392] - Mouse spleen stained wi...
Western Blotting (WB) image for anti-Forkhead Box P3 (FOXP3) antibody (ABIN188539) Western Blot: FOXP3 Antibody [ABIN1885392] - Jurkat Tag cells were transfected with t...
Immunofluorescence (IF) image for anti-Forkhead Box P3 (FOXP3) antibody (ABIN188539) Immunocytochemistry/Immunofluorescence: FOXP3 Antibody [ABIN1885392] - The same trans...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Forkhead Box P3 (FOXP3) antibody (ABIN188539) Immunohistochemistry-Paraffin: FoxP3 Antibody - IHC analysis of a formalin fixed par...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Forkhead Box P3 (FOXP3) antibody (ABIN188539) Immunohistochemistry-Paraffin: FoxP3 Antibody - IHC analysis of a formalin fixed par...
Western Blotting (WB) image for anti-Forkhead Box P3 (FOXP3) antibody (ABIN188539) Western Blot: FoxP3 Antibody - Detection of immunoprecipitated FOXP3 from HA-tagged ...
Produit citée dans: Wang, Sun, Xiao, Feng, Mikule, Ma, Feng, Vellano, Federico, Marszalek, Mills, Hanke, Ramaswamy, Wang: "Niraparib activates interferon signaling and potentiates anti-PD-1 antibody efficacy in tumor models." dans: Scientific reports, Vol. 9, Issue 1, pp. 1853, 2019 (PubMed). Méthode utilisée: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Échantillon (espèces): Human).

Sun, Lian, Ma, Wang, Ma, Wang, Zhai, Meng, Feng, Bai, Cui, Fang: "Competing endogenous RNA network analysis of CD274, IL‑10 and FOXP3 co‑expression in laryngeal squamous cell carcinoma." dans: Molecular medicine reports, Vol. 17, Issue 3, pp. 3859-3869, 2018 (PubMed). Méthode utilisée: Immunohistochemistry (IHC)

Samanta, Park, Ni, Li, Zahnow, Gabrielson, Pan, Semenza: "Chemotherapy induces enrichment of CD47+/CD73+/PDL1+ immune evasive triple-negative breast cancer cells." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, Issue 6, pp. E1239-E1248, 2018 (PubMed).

Petrizzo, Mauriello, Luciano, Rea, Barbieri, Arra, Maiolino, Tornesello, Gigantino, Botti, Ciliberto, Buonaguro, Tagliamonte, Buonaguro: "Inhibition of tumor growth by cancer vaccine combined with metronomic chemotherapy and anti-PD-1 in a pre-clinical setting." dans: Oncotarget, Vol. 9, Issue 3, pp. 3576-3589, 2018 (PubMed).

Yu, Bhattacharya, Yanamandra, Kilian, Shi, Yadavilli, Katlinskaya, Kaczynski, Conner, Benson, Hahn, Seestaller-Wehr, Bi, Vitali, Tsvetkov, Halsey, Hughes, Traini, Zhou, Jing, Lee, Figueroa, Brett et al.: "Tumor-immune profiling of murine syngeneic tumor models as a framework to guide mechanistic studies and predict therapy response in distinct tumor microenvironments. ..." dans: PLoS ONE, Vol. 13, Issue 11, pp. e0206223, 2018 (PubMed). Méthode utilisée: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Échantillon (espèces): Mouse (Murine)).

Jiang, Cao, Ma, Wang, Wang, Wang, Yang, Zhao, Liu, Li, Feng: "Auricular Vagus Nerve Stimulation Exerts Antiinflammatory Effects and Immune Regulatory Function in a 6-OHDA Model of Parkinson's Disease." dans: Neurochemical research, Vol. 43, Issue 11, pp. 2155-2164, 2018 (PubMed). Méthode utilisée: Immunofluorescence (fixed cells) (IF/ICC) (Échantillon (espèces): Rat (Rattus)).

Schmidt, Solimani, Pollmann, Stein, Schmidt, Stulberg, Kühn, Eming, Eubel, Kind, Arweiler, Sitaru, Hertl: "TH1/TH17 cell recognition of desmoglein 3 and bullous pemphigoid antigen 180 in patients with lichen planus." dans: The Journal of allergy and clinical immunology, Vol. 142, Issue 2, pp. 669-672.e7, 2018 (PubMed). Méthode utilisée: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Échantillon (espèces): Human).

Busch, Annamalai, Abdusalamova, Reichhart, Huber, Lin, Jo, Zipfel, Skerka, Wildner, Diedrichs-Möhring, Rohrer, Strauß: "Anaphylatoxins Activate Ca(2+), Akt/PI3-Kinase, and FOXO1/FoxP3 in the Retinal Pigment Epithelium." dans: Frontiers in immunology, Vol. 8, pp. 703, 2017 (PubMed). Méthode utilisée: Immunofluorescence (fixed cells) (IF/ICC) (Échantillon (espèces): Rat (Rattus)).

Varga, Piazuelo, Romero-Gallo, Delgado, Suarez, Whitaker, Krishna, Patel, Skaar, Wilson, Algood, Peek: "TLR9 activation suppresses inflammation in response to Helicobacter pylori infection." dans: American journal of physiology. Gastrointestinal and liver physiology, Vol. 311, Issue 5, pp. G852-G858, 2016 (PubMed). Méthode utilisée: Immunohistochemistry (IHC)

Zhang, Yao, Wang, Yang, Wang, Fan, Harris: "Inhibition of cyclooxygenase-2 in hematopoietic cells results in salt-sensitive hypertension." dans: The Journal of clinical investigation, Vol. 125, Issue 11, pp. 4281-94, 2015 (PubMed).

Kreiter, Vormehr, van de Roemer, Diken, Löwer, Diekmann, Boegel, Schrörs, Vascotto, Castle, Tadmor, Schoenberger, Huber, Türeci, Sahin: "Mutant MHC class II epitopes drive therapeutic immune responses to cancer." dans: Nature, Vol. 520, Issue 7549, pp. 692-6, 2015 (PubMed). (Échantillon (espèces): Human). Détails: Immunohistochemistry (Paraffin-embedded Sections)

Rajnai, Heyning, Koens, Sebestyén, Andrikovics, Hogendoorn, Matolcsy, Szepesi: "The density of CD8+ T-cell infiltration and expression of BCL2 predicts outcome of primary diffuse large B-cell lymphoma of bone." dans: Virchows Archiv : an international journal of pathology, Vol. 464, Issue 2, pp. 229-39, 2014 (PubMed). (Échantillon (espèces): Human). Détails: Immunohistochemistry (Paraffin-embedded Sections)

Grant, Jung, Johnson, Kostakoglu, Hsi, Byrd, Jones, Leonard, Martin, Cheson: "A phase 2 trial of extended induction epratuzumab and rituximab for previously untreated follicular lymphoma: CALGB 50701." dans: Cancer, Vol. 119, Issue 21, pp. 3797-804, 2013 (PubMed). (Échantillon (espèces): Human). Détails: Immunohistochemistry