GAD65 anticorps (AA 4-22)

N° du produit ABIN1897298

Détail du produit anti-GAD65 anticorps

Antigène: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Épitope: AA 4-22

Reactivité: Humain, Rat, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GAD65 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)

Marque: IHC-plus™

Specificité: Recognizes an epitope within the N-terminal (NT) region of Glutamate decarboxylase 2, otherwise known as GAD65/GAD2, an amphiphilic membrane-anchored protein and member of the group 2 decarboxylase family, principally expressed in the brain and also in pancreatic beta cells. GAD65 catalyzes the decarboxylation of glutamate to GABA, the major inhibitory neurotransmitter in the central nervous system. GAD65 is the 65kD isoform of GAD, encoded by the GAD2 gene, which is predominantly expressed by nerve termini, as oppose to the 67kD isoform (GAD67), which is predominantly found in the cell body, and is encoded by the GAD1 gene. Studies have shown the presence of autoantibodies to GAD65 (GAD65Ab) in autoimmune diseases, including Graves disease and Stiff Man Syndrome (SMS), but GAD65Ab are most prevalent in patients with Type I diabetes mellitus, and those at high risk of developing Type I diabetes. The N-Terminal region of GAD65 lacks the epitopes for GAD65Ab in Type I diabetes patients, and has been shown as essential for targeting the enzyme to GABA-containing secretory vesicles. Clone N-GAD65 has been shown to be highly specific for GAD65 and does not recognize GAD67.

Purification: Protein G purified

Immunogène: Keyhole Limpet Hemocyanin (KLH) conjugated synthetic peptide sequence PGSGFWSFGSEDGSGDSEN corresponding to amino acids 4-22 within the N-terminal region of human GAD65. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan (100%), Gibbon, Galago, Rat, Ferret, Panda, Bovine, Cat, Dog, Horse, Pig (95%), Monkey, Marmoset, Mouse, Rabbit, Opossum, Guinea pig (89%).

Type of Immunogen: Synthetic peptide

Clone: N-GAD65

Isotype: IgG1

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), IP, RIA, WB (1:100 - 1:1000)

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles.

Détails sur GAD65

Antigène: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Autre désignation: GAD65 (GAD2 Produits)

Synonymes: anticorps GAD65, anticorps gad65, anticorps F12P19.12, anticorps F12P19_12, anticorps GLUTAMATE DECARBOXYLASE 2, anticorps glutamate decarboxylase 2, anticorps zgc:112198, anticorps 6330404F12Rik, anticorps GAD(65), anticorps Gad-2, anticorps glutamate decarboxylase 2, anticorps glutamate decarboxylase 1, anticorps glutamic acid decarboxylase 2, anticorps GAD2, anticorps PTRG_06148, anticorps LOC9324534, anticorps Gad2, anticorps GAD1, anticorps gad2

Sujet: Name/Gene ID: GAD2

Synonyms: GAD2, GAD-65, Glutamate decarboxylase 2, GAD65

ID gène: 2572