NMDAR1 Antibody detects endogenous levels of mouse NMDAR1. It is predicted to react with human and rat NMDAR1 protein according to sequence homology.Positive Control: Mouse brain
Réactivité croisée (Details)
NMDAR1 Antibody detects endogenous levels of mouse NMDAR1. It is predicted to react with human and rat NMDAR1 protein according to sequence homology.Positive Control: Mouse brain
Purification
Immunoaffinity chromatography
Immunogène
KLH-coupled synthetic peptide within AA 900-950 of human NMDAR1
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. Western blot: 0.5-1 µg/mLFlow cytometry: 1-3 µg for 1 x 106 cellsOther applications: user-optimized
Restrictions
For Research Use only
Format
Lyophilized
Buffer
PBS, pH 7.4, containing 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Stock
4 °C/-20 °C
Stockage commentaire
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
N-methyl-D-aspartate receptor (NMDAR) forms a heterodimer of at least one NMDAR1 and one NR2A-D subunit. NMDAR1 subunits and NR2 subunits bind to the co-agonist glycine and the neurotransmitter glutamate, respectively.'PKC'an phosphorylate NMDAR1 of the receptor at Ser890/Ser896, and PKA can phosphorylate NMDAR1 at Ser897. The phosphorylation of NMDAR1 by'PKC'ecreases its affinity for calmodulin, thus preventing the inhibitory effect of calmodulin on NMDAR. The phosphorylation of NMDAR1 by PKA probably counteracts the inhibitory effect of calcineurin on the receptor. NMDAR1 Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 900-950 of human NMDAR1 (Swiss Prot: Q05586).