Integrin Alpha2b anticorps (Biotin)

N° du produit ABIN2144311

Détail du produit anti-Integrin Alpha2b anticorps (Biotin)

Antigène: Integrin Alpha2b (CD41)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Integrin Alpha2b est conjugé à/à la Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Attributs du produit: 4AHIP8 reacts with a calcium-dependent complex of CD41/CD61 ( GPIIb/IIIa), 135/120 kDa, expressed on normal platelets and megakaryocytes.

Clone: 4AHIP8

Isotype: IgG1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Procédure de l'essai:

• Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
• Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature,
• Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
• Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 2 mLPBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature.
• Repeat step 5.
• Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détails sur Integrin Alpha2b

Antigène: Integrin Alpha2b (CD41)

Autre désignation: CD41 (CD41 Produits)

Synonymes: anticorps AI172977, anticorps CD41, anticorps CD41B, anticorps GpIIb, anticorps alphaIIb, anticorps BDPLT16, anticorps BDPLT2, anticorps GP2B, anticorps GPIIb, anticorps GT, anticorps GTA, anticorps HPA3, anticorps integrin alpha 2b, anticorps integrin subunit alpha 2b, anticorps Itga2b, anticorps ITGA2B

Sujet: CD41 antigen acts as a receptor for fibrinogen, von Willebrand factor (vWf), fibrinectin and vitronectin and mediates platelet adhesion and aggregation. GM1CD41 antibody completely inhibits ADP, epinephrine and collagen-induced platelet activation and partially inhibits restocetin and thrombin-induced platelet activation. It is useful in the morphological and physiological studies of platelets and megakaryocytes. Application: Diagnosis of Glanzmann's Thrombasthenia and megakaryoblastic leukemias, deletion of alloantibodies, and anti-thrombotic therapy.

Pathways: Integrin Complex