CD5 anticorps (FITC)

N° du produit ABIN2144469

Détail du produit anti-CD5 anticorps (FITC)

Antigène: CD5

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD5 est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Cytometry by Time of Flight (CyTOF)

Attributs du produit: 4AUCHT2 reacts with 58 kDa molecule, a member of the scavenger receptor superfamily, expressed on thymocytes and all mature T lymphocytes. It also expressed on a small subset of mature B lymphocytes ( B1a cells ) which is expanded during fetal life, and in several autoimmune disorders, as well as in some B-CLL .CD5 may serve as a dual receptor which provides inhibitiry signals in thymocytes and B1a cells and acts as a costimulatory signal receptor. CD5-mediated cellular interaction may influence thymocyte maturation and selection. CD5 is a phenotypic marker for some B-cell lymphoproliferative disorders (B-CLL, mantle zone lymphoma , hairy cell leukemia, etc). The increase of blood CD3+/CD5- T cells correlates with the presence of GVHD.

Clone: 4AUCHT2

Isotype: IgG1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Procédure de l'essai:

• Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
• Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
• Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
• Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détails sur CD5

Antigène: CD5

Autre désignation: CD5 (CD5 Produits)

Synonymes: anticorps CD5, anticorps Ly-1, anticorps Ly-12, anticorps Ly-A, anticorps Lyt-1, anticorps LEU1, anticorps T1, anticorps CD5 molecule, anticorps CD5 antigen, anticorps CD5 molecule like, anticorps Cd5 molecule, anticorps CD5, anticorps Cd5, anticorps CD5L