CD58 anticorps (FITC)
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- Antigène Voir toutes CD58 Anticorps
- CD58
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD58 est conjugé à/à la FITC
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Application
- Flow Cytometry (FACS)
- Attributs du produit
- 4ATS2/9.1 reacts with 55-70 kDa CD58, lymphocyte function-associated antigen (LFA-3). It is expressed in hematipoietic and non-hematopoietic tissue including leukocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts.CD58 mediates adhesion between killer and target cells, antigen presenting cells and T cells, thymocytes and thymic epithelial cells. It also provides co-stimulatory signal in immune response.
- Clone
- 4ATS2-9-1-1-1
- Isotype
- IgG1
- Top Product
- Discover our top product CD58 Anticorps primaire
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Procédure de l'essai
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
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- Antigène
- CD58
- Autre désignation
- CD58 (CD58 Produits)
- Synonymes
- anticorps LFA-3, anticorps LFA3, anticorps ag3, anticorps CD58 molecule, anticorps CD58
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