Reactivité: Humain, Souris, Rat
ELISA, IF, WB
This antibody reacts with human APC11 by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins (APC2) does occur. A 9.8 kDa band corresponding to human APC11 is detected. Most cell lines or tissues expressing APC11 can be used as a positive control. Researchers should determine optimal titers for other applications.
For Research Use only
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Furukawa, Ohta, Xiong: "Activation of UBC5 ubiquitin-conjugating enzyme by the RING finger of ROC1 and assembly of active ubiquitin ligases by all cullins." dans: The Journal of biological chemistry, Vol. 277, Issue 18, pp. 15758-65, (2002) (PubMed).
Ohta, Michel, Xiong: "Association with cullin partners protects ROC proteins from proteasome-dependent degradation." dans: Oncogene, Vol. 18, Issue 48, pp. 6758-66, (2000) (PubMed).
APC11 is also known as Anaphase promoting complex subunit 11, APC11, Cyclosome subunit 11, Hepatocellular carcinoma associated RING finger protein, and HSPC214. APC11 is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC11 may function to recruit the E2 ubiquitin-conjugating enzymes to the complex. APC11 interacts with the cullin domain of ANAPC2 and also interacts with UBE2D2. APC11 shows both a cytoplasmic and nuclear localization. APC11 is expressed at high levels in skeletal muscle and heart; in moderate levels in brain, kidney, and liver; and at low levels in colon, thymus, spleen, small intestine, placenta, lung and peripheral blood leukocyte. APC11 is a member of the RING-type zinc finger family and is auto-ubiquitinylated. Synonyms: Anaphase promoting complex subunit 11 homolog antibody, APC11 anaphase promoting complex subunit 11 homolog antibody, Cyclosome subunit 11 antibody Hepatocellular carcinoma associated RING finger protein antibody HSPC 214 antibody