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SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8) anticorps

SARS-CoV NSP8 Reactivité: SARS Coronavirus (SARS-CoV) FM, IEM, IP, WB Hôte: Lapin Polyclonal unconjugated
Pubmed (2 références)
N° du produit ABIN233792
$586.60
Plus frais de port $45.00 et $20.00 pour carboglace
100 μL
Destination: Etats-Unis
Envoi sous 1 à 2 jours ouvrables
  • Antigène Voir toutes SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8) Anticorps
    SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8)
    Reactivité
    SARS Coronavirus (SARS-CoV)
    Hôte
    • 2
    Lapin
    Clonalité
    • 1
    • 1
    Polyclonal
    Application
    Fluorescence Microscopy (FM), Immunoelectron Microscopy (IEM), Immunoprecipitation (IP), Western Blotting (WB)
    Attributs du produit
    Concentration Definition: by Refractometry
    Purification
    Antiserum
    Immunogène
    This whole rabbit serum was produced by repeated immunizations with a purified His- tagged recombinant protein corresponding to full-length SARS-Coronavirus nsp8.
    Immunogentype:Recombinant
  • Indications d'application
    This antibody has been tested for use in immunofluorescence microscopy, immunoelectron microscopy, immunoprecipitation and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 22 kDa in size corresponding to SARS-CoV nsp8 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37°C.  Infection occurred in PBS/DEAE/2%FCS followed by exchange to EMEM/25mMHEPES/2%FCS. Cells were fixed with PBS/3%PFA.  After washing fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    85 mg/mL
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
  • Ng, Faulkner, Finsterbusch, Wu, Harvey, Hussain, Greco, Liu, Kjaer, Swanton, Gandhi, Beale, Gamblin, Cherepanov, McCauley, Daniels, Howell, Arase, Wack, Bauer, Kassiotis: "SARS-CoV-2 S2-targeted vaccination elicits broadly neutralizing antibodies." dans: Science translational medicine, Vol. 14, Issue 655, pp. eabn3715, (2022) (PubMed).

    Tan, Rijal, Rahikainen, Keeble, Schimanski, Hussain, Harvey, Hayes, Edwards, McLean, Martini, Pedrera, Thakur, Conceicao, Dietrich, Shelton, Ludi, Wilsden, Browning, Zagrajek, Bialy, Bhat et al.: "A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV-2 spike protein receptor-binding domain induces potent neutralising antibody responses. ..." dans: Nature communications, Vol. 12, Issue 1, pp. 542, (2021) (PubMed).

  • Antigène
    SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8)
    Abstract
    SARS-CoV NSP8 Produits
    Synonymes
    pp1a, pp1ab, orf1ab polyprotein (pp1ab), orf1ab
    Classe de substances
    Viral Protein
    Sujet
    The nonstructural protein 8 (nsp8) is one of the SARS-Coronavirus replicase cleaving products, encoded by ORF1a. Nsp8 is thought to be part of the viral replication complex, which is associated with intracellular membranes.  No specific information on the function of nsp8 is available.
    Synonyms: Replicase polyprotein 1a, ORF1a polyprotein, nsp3, PL2-PRO, Papain-like proteinase, PL-PRO antibody, SARS coronavirus main proteinase, nsp4, 3CL-PRO, nsp5, nsp6, nsp7, nsp8, nsp9, nsp10, GFL, nsp11
    ID gène
    1489680, 30124074
    NCBI Accession
    NP_828866
    UniProt
    P0C6U8
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