LAT2 anticorps (AA 91-243)

N° du produit ABIN238343

Détail du produit anti-LAT2 anticorps

Antigène: LAT2 (Linker For Activation of T Cells Family, Member 2 (LAT2))

Épitope: AA 91-243

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LAT2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: The polyclonal antibody recognizes the defined epitope (aa 91-243) of human Non-T cell Activation Linker (NTAL), also known as LAB (linker of activated B cells), a 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B cells, NK cells and myeloid cells.

Purification: Protein-A affinity chromatography.

Immunogène: Recombinant cytoplasmic domain (aa 91-243) of human NTAL.

Information d'application

Indications d'application: Suitable for Western blot under Non-Reducing conditions: 15 % separating SDS-PAGE gel. Recommended dilution: 1 μg/mL, incubation 60 min in RT, or overnight at 4 °C. Positive control: RAMOS human lymphoma cell line. THP-1 human monocytic leukemia cell line. Negative control: JURKAT human T cell leukemia cell line. Sample preparation: Resuspend approx.50 mil. cells in 1 mLcold Lysis buffer (1 %laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysatewith non-reducing SDS-PAGE sample buffer. Immunohistochemistry on paraffin sections: 10 μg/mL. Positive tissue: spleen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 with 15 mM sodium azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur LAT2

Antigène: LAT2 (Linker For Activation of T Cells Family, Member 2 (LAT2))

Autre désignation: LAT2 / WBSCR15 (LAT2 Produits)

Synonymes: anticorps LAB, anticorps NTAL, anticorps WBSCR15, anticorps WBSCR5, anticorps WSCR5, anticorps LAT2, anticorps MGC139435, anticorps Ntal, anticorps Wbscr5, anticorps AW125574, anticorps Wbscr15, anticorps linker for activation of T-cells family member 2, anticorps linker for activation of T cells family, member 2, anticorps LAT2, anticorps Lat2

Sujet: NTAL (Non-T cell activation linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.Synonyms: HSPC046, LAB, LLinker for activation of B-cells, Linker for activation of T-cells family member 2, Membrane-associated adapter molecule, NTAL, Non-T-cell activation linker, WBS15, WBSCR5, Williams-Beuren syndrome chromosomal region 15 protein

ID gène: 7462

NCBI Accession: NP_054865

UniProt: Q9GZY6

Pathways: Fc-epsilon Receptor Signaling Pathway, BCR Signaling