NSUN2 anticorps

N° du produit ABIN2459234

Détail du produit anti-NSUN2 anticorps

Antigène: NSUN2 (NOP2/Sun Domain Family, Member 2 (NSUN2))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NSUN2 est non-conjugé

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogène: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NSUN2.

Information d'application

Indications d'application: NSUN2 antibody can be used for detection of NSUN2 by ELISA at 1:62500. NSUN2 antibody can be used for detection of NSUN2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Conseil sur la manipulation: As with any antibody avoid repeat freeze-thaw cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: For short periods of storage (days) store at 4 °C. For longer periods of storage, store NSUN2 antibody at -20 °C.

Détails sur NSUN2

Antigène: NSUN2 (NOP2/Sun Domain Family, Member 2 (NSUN2))

Autre désignation: NSUN2 (NSUN2 Produits)

Synonymes: anticorps D13Wsu123e, anticorps Misu, anticorps MISU, anticorps MRT5, anticorps SAKI, anticorps TRM4, anticorps RGD1311954, anticorps NOL1/NOP2/Sun domain family member 2, anticorps NOP2/Sun RNA methyltransferase family member 2, anticorps NOP2/Sun RNA methyltransferase family, member 2, anticorps NOP2/Sun RNA methyltransferase family member 2 S homeolog, anticorps Nsun2, anticorps NSUN2, anticorps nsun2.S

Sujet: Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu (CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 is a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu (CAA).Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu (CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu (CAA) (Brzezicha et al., 2006 [PubMed 17071714]).

Poids moléculaire: 86 kDa

ID gène: 54888

NCBI Accession: NP_060225

UniProt: Q08J23