Interleukin 6 anticorps (IL6)

Details for Product anti-IL6 Antibody No. ABIN2469708
Antigène
Reactivité
Humain
444
197
116
23
17
16
12
10
9
9
7
7
6
4
1
1
Hôte
Souris
402
225
114
36
7
5
4
Clonalité (Clone)
Monoclonal ()
Conjugué
Cet anticorp Interleukin 6 est non-conjugé
110
35
17
15
12
7
7
7
7
7
7
7
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
540
414
172
124
110
67
57
53
45
43
41
35
32
24
10
8
8
7
5
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Options
'Independent Validation' signe
  • Testé avec succès (Immunohistochemistry)
  • by  HIK Hannover
  • No. #100788
  • Date 31.05.2017
  •  
Antigène IL6
Numéro du lot 19346-1701
Application validée Immunohistochemistry
Contrôle positif Formalin fixed and plastic embedded human liver and tonsil sections
Contrôle négative No primary antibody
Conclusion Passed. The IL6 antibody ABIN2469708 specifically labels the targeted antigen in human liver and tonsil tissue sections in IHC. No signal was detected in no primary antibody negative controls.
Anticorps primaire ABIN2469708
Anticorps secondaire Histofine Simple Stain MAX PO (M) Anti-Mouse (Medac Diagnostika, 414132F, lot no H1608)
Protocole
  • Disect tissue and fix in 4% buffered formalin for >24h at RT.
  • Plastification in Technovit 9100 plastic embedding system (Heraeus Kulzer) according to manufactuer’s recommencations.
  • Sectioning into 5µm sections on a rotary microtome (Reichert Jung, 1140/Autocut).
  • Deplastify and rehydrate tissue through graded xylene and graded alcohol series:
    • xylene for 20min.
    • MEA (2-Methoxyethyl)acetat zur Synthese (Merck, 8.06061.1000) 2x 20min.
    • 100% ethanol for 1min.
    • 95% ethanol for 1min.
    • 70% ethanol for 1min.
    • H2O for 2min.
    • distilled H2O 2min.
  • Heat induced epitope retrieval in Antigen Retrieval Buffer 4 pH9 (Medac Diagnostika, PMB4-125, lot 161109AB) ON at 60°C in an oven (Memmert, UNB 200).
  • Quench sections in in ice cold distilled H2O 2min.
  • Adjust sections with wash buffer (Medac Diagnostika, B1-30A, lot no161102001) 5min at RT.
  • Incubate sections with primary Interleukin 6 antibody (IL6) (antibodies-online, ABIN2469708, lot 19346-1701) diluted 1:200 in dilution buffere (Medac Diagnostika, B1-31C, lot no 161205001) for 30min at RT. Include no primary antibody negative controls.
  • Rinse sections 3x 5min with wash buffer. Keep negative controls in a separate container.
  • Block endogenous peroxidase with Peroxide Block RTU (Medac Diagnostika, 925B-09, lot no 161103001) for 10min at RT.
  • Rinse sections 3x 5min with wash buffer.
  • Incubate sections with Histofine Simple Stain MAX PO (M) Anti-Mouse (Medac Diagnostika, 414132F, lot no H1608) for 30min at RT.
  • Rinse sections 3x 5min with wash buffer.
  • Incubate sections with Bright DAB (Medac Diagnostika, BS04-110,lot no 160523) for 10min at RT.
  • Rinse sections in distilled H2O.
  • Counterstain with Mayers Haematoxylin (Leica, 3801582E, lot no 3801582E) 5min at RT.
  • Dehydrate in fresh ethanol:
    • 95% ethanol twice for 2min.
    • 99.5% ethanol twice for 2min.
    • Xylene twice for 2min.
  • Mount sections with Celltexx mounting medium (Engelbrecht, 19360, lot no 1716047).
  • Dry sections in a ventilated place.
  • Image acquisition on a Leica Microsystems DM 1000 light microscope with a DFC 320 digital camera. Magnification tonsil sections 40x, liver sections 10x and 20x.
Notes The immunostaining pattern of human liver and tonsil with ABIN2469708 is consistent with published data.
Validation Images
Immunohistochemistry validation image for anti-Interleukin 6 (IL6) antibody (ABIN2469708) Immunostaining of IL6 in human liver liver (A) and tonsil sections (C) using ABIN2469...
Immunogène IL - 6 monoclonal antibody was raised against Ni-NTA purified recombinant human IL-6 expressed in E. Coli strain BL21 (DE3)
Clone 6D9A1-6C12B1
Isotype IgG1
Purification Ascites
Antigène
Autre désignation IL-6 (IL6 Antibody Extrait)
ID gène 3569
UniProt P05231
Pathways Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints
Indications d'application IL-6 antibody can be used in ELISA starting at 1/200 - 1/800, and immunohistochemistry starting at 10 mg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid, 0.03 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation As with all antibodies avoid freeze/thaw cycles.
Stock 4 °C/-20 °C
Stockage commentaire Store IL-6 antibody at 4 °C or -20 °C.
Images (Fournisseur)
Image no. 1 for anti-Interleukin 6 (IL6) antibody (ABIN2469708) anti-Interleukin 6 (IL6) antibody
Produit citée dans: Ingendoh-Tsakmakidis, Mikolai, Winkel, Szafrański, Falk, Rossi, Walles, Stiesch: "Commensal and pathogenic biofilms differently modulate peri-implant oral mucosa in an organotypic model." dans: Cellular microbiology, pp. e13078, 2019 (PubMed).