anticorps CBR, anticorps SDR21C1, anticorps hCBR1, anticorps OPLL, anticorps AI747156, anticorps Col6a-1, anticorps RGD1565398, anticorps COL6A1, anticorps carbonyl reductase 1, anticorps collagen type VI alpha 1 chain, anticorps collagen, type VI, alpha 1, anticorps collagen, type VI, alpha 1 L homeolog, anticorps collagen type VI alpha 3 chain, anticorps collagen alpha-1(VI) chain, anticorps CBR1, anticorps COL6A1, anticorps Col6a1, anticorps col6a1.L, anticorps COL6A3, anticorps col6a1, anticorps LOC100623720
Sujet
Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.