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Ubiquitin anticorps (Biotin)

Ubiquitin Reactivité: Boeuf (Vache) WB, IHC, IF, ICC, IP Hôte: Lapin Polyclonal Biotin
N° du produit ABIN2481455
  • Antigène Voir toutes Ubiquitin Anticorps
    Ubiquitin
    Reactivité
    • 148
    • 70
    • 70
    • 68
    • 18
    • 18
    • 16
    • 15
    • 14
    • 14
    • 14
    • 14
    • 13
    • 12
    • 11
    • 10
    • 6
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Boeuf (Vache)
    Hôte
    • 108
    • 73
    • 3
    • 2
    • 1
    • 1
    Lapin
    Clonalité
    • 100
    • 87
    Polyclonal
    Conjugué
    • 81
    • 15
    • 13
    • 12
    • 12
    • 11
    • 7
    • 7
    • 7
    • 7
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Ubiquitin est conjugé à/à la Biotin
    Application
    • 178
    • 104
    • 95
    • 69
    • 59
    • 42
    • 27
    • 22
    • 21
    • 14
    • 13
    • 10
    • 4
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)
    Specificité
    Detects ~10 kDa. It also recognizes ubiquinated proteins.
     Réactivité croisée
    Poulet, Boeuf (Vache), Chien, Drosophila melanogaster, Poisson, Cobaye, Hamster, Humain, Singe, Souris, Porc, Lapin, Truite arc-en-ciel, Rat, Saccharomyces cerevisiae, Salmon, Mouton, Xenopus laevis
    Purification
    Peptide Affinity Purified
    Immunogène
    Native bovine Ubiquitin, conjugated to KLH
    Top Product
    Discover our top product Ubiquitin Anticorps primaire
  • Indications d'application
    • WB (1:1000)
    • IHC (1:100)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.
    Commentaires

    A 1:1000 dilution of ABIN2481455 was sufficient for detection of free ubiquitin in 15 μg of HeLa lysate by ECL immunoblot analysis using Donkey anti-rabbit IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Conjugated antibodies should be stored at 4°C
  • Antigène
    Ubiquitin
    Autre désignation
    Ubiquitin (Ubiquitin Produits)
    Synonymes
    anticorps CG11624, anticorps DmUb, anticorps DmUbi-p63E, anticorps Dmel\\CG11624, anticorps Ub, anticorps Ubi-P63e, anticorps Ubi-p, anticorps Ubi63E, anticorps Ubi63p, anticorps Ubip, anticorps Ubiq, anticorps Ubp, anticorps ubi, anticorps ubi-p63E, anticorps ubi63E, anticorps CG18282, anticorps CG32744, anticorps CR32744, anticorps DmUbi-p5E, anticorps Dmel\\CG32744, anticorps ubiquitin, anticorps si:dz180g5.5, anticorps si:busm1-180g5.5, anticorps LOC100194618, anticorps UBIQ, anticorps Ubiquitin, anticorps LOC100282068, anticorps Ubiquitin-63E, anticorps Ubiquitin-5E, anticorps transmembrane and ubiquitin-like domain containing 2, anticorps polyubiquitin, anticorps Putative ubiquitin family protein, anticorps ubiquitin, anticorps ubiquitin B, anticorps UBiQuitin, anticorps Ubiquitin, anticorps Ubi-p63E, anticorps Ubi-p5E, anticorps tmub2, anticorps LOC100194618, anticorps pco110026, anticorps LOC100135798, anticorps UBB, anticorps ubq-1, anticorps LOC100190766
    Sujet
    Ubiquitin is a small protein that occurs in all eukaryotic cells. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Key features include its C-terminal tail and the 7 Lys residues. It is highly conserved among eukaryotic species: Human and yeast ubiquitin share 96 % sequence identity (1). The main function of Ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome (2). Ubiquitination represents an essential cellular process affected by a multi-enzyme cascade involving classes of enzymes known as ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs) and ubiquitin-protein ligases (E3s). Ubiquitin is activated in a two-step reaction by an E1 ubiquitin-activating enzyme in a process requiring ATP as an energy source. The initial step involves production of an ubiquitin-adenylate intermediate. The second step transfers ubiquitin to the E1 active site cysteine residue, with release of AMP. This step results in a thioester linkage between the C-terminal carboxyl group of ubiquitin and the E1 cysteine sulfhydryl group. The third step is a transfer of ubiquitin from E1 to the active site cysteine of a ubiquitin-conjugating enzyme E2 via a trans(thio)esterification reaction. And the final step of the ubiquitylation cascade creates an isopeptide bond between a lysine of the target protein and the C-terminal glycine of ubiquitin. In general, this step requires the activity of one of the hundreds of E3 ubiquitin-protein ligases (often termed simply ubiquitin ligase). E3 enzymes function as the substrate recognition modules of the system and are capable of interaction with both E2 and substrate(3, 4). Ubiquitination also participates in the internalization and degradation of plasma membrane proteins such as some of the TCR subunits while still ER-membrane associated (5). Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IκBα and p27 (6).
    ID gène
    281370
    NCBI Accession
    NP_776558
    UniProt
    P0CG53
    Pathways
    Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
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