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GRP94 anticorps (C-Term) (PE)

HSP90B1 Reactivité: Souris WB, IHC, IF, IP, ICC Hôte: Lapin Polyclonal PE
N° du produit ABIN2481972
  • Antigène Voir toutes GRP94 (HSP90B1) Anticorps
    GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))
    Épitope
    • 21
    • 18
    • 16
    • 7
    • 7
    • 7
    • 7
    • 7
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivité
    • 133
    • 85
    • 55
    • 40
    • 35
    • 31
    • 30
    • 30
    • 28
    • 28
    • 26
    • 26
    • 25
    • 17
    • 10
    • 2
    • 2
    • 1
    • 1
    Souris
    Hôte
    • 92
    • 40
    • 24
    • 2
    • 1
    Lapin
    Clonalité
    • 93
    • 66
    Polyclonal
    Conjugué
    • 70
    • 12
    • 11
    • 11
    • 9
    • 8
    • 4
    • 4
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp GRP94 est conjugé à/à la PE
    Application
    • 151
    • 83
    • 68
    • 62
    • 49
    • 45
    • 38
    • 21
    • 13
    • 13
    • 12
    • 11
    • 4
    • 4
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Specificité
    Detects ~94 kDa.
     Réactivité croisée
    Boeuf (Vache), Humain, Souris, Rat
    Purification
    Peptide Affinity Purified
    Immunogène
    Synthetic peptide corresponding to the sequence near the C-terminus of mouse GRP94
    Top Product
    Discover our top product HSP90B1 Anticorps primaire
  • Indications d'application
    • WB (1:1000)
    • ICC/IF (1:120)
    • IP (1:80)
    • optimal dilutions for assays should be determined by the user.
    Commentaires

    1 μg/ml of ABIN2481972 was sufficient for detection of Grp94 in 20 μg of Hela lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Conjugated antibodies should be stored at 4°C
  • Antigène
    GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))
    Autre désignation
    GRP94 (HSP90B1 Produits)
    Synonymes
    anticorps ecgp, anticorps gp96, anticorps tra1, anticorps grp94, anticorps DDBDRAFT_0206450, anticorps DDBDRAFT_0215015, anticorps DDB_0206450, anticorps DDB_0215015, anticorps TRA1, anticorps HSP90B1, anticorps ERp99, anticorps GRP94, anticorps TA-3, anticorps Targ2, anticorps Tra-1, anticorps Tra1, anticorps endoplasmin, anticorps GP96, anticorps fb61d09, anticorps tra-1, anticorps wu:fb61d09, anticorps wu:fq25g01, anticorps Grp94, anticorps ECGP, anticorps Gp96, anticorps ppk98, anticorps hsp108, anticorps heat shock protein 90kDa beta (Grp94), member 1, anticorps heat shock protein Hsp90 family protein, anticorps 94 kD glucose-regulated protein, anticorps heat shock protein 90 beta family member 1, anticorps heat shock protein 90, beta (Grp94), member 1, anticorps heat shock protein 90, beta (grp94), member 1, anticorps heat shock protein 90kDa beta family member 1 L homeolog, anticorps hsp90b1, anticorps grp94, anticorps HSP90B1, anticorps Hsp90b1, anticorps hsp90b1.L
    Sujet
    Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion (1). Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway (2). Grp94 is a member of the HSP90 family of stress proteins and shares sequence homology with its cytosolic equivalent, HSP90 (3). Both HSP90 and Grp94 are calcium binding proteins (4). Despite sharing 50 % sequence homology over its N domains and complete conservation in its ligand binding domains with HSP90, Grp94 and HSP90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolyisis activity (5). Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits. (6). Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER (7).
    ID gène
    22027
    NCBI Accession
    NP_035761
    UniProt
    P08113
    Pathways
    Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades
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