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CAMKII gamma anticorps (HRP)

CAMK2G Reactivité: Rat WB, IHC, ELISA, IP, IF, ICC, RIA Hôte: Souris Monoclonal 6G9 HRP
N° du produit ABIN2484561
  • Antigène Voir toutes CAMKII gamma (CAMK2G) Anticorps
    CAMKII gamma (CAMK2G) (Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G))
    Reactivité
    • 69
    • 60
    • 46
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Rat
    Hôte
    • 54
    • 31
    • 1
    Souris
    Clonalité
    • 49
    • 37
    Monoclonal
    Conjugué
    • 41
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp CAMKII gamma est conjugé à/à la HRP
    Application
    • 57
    • 35
    • 31
    • 25
    • 21
    • 14
    • 10
    • 5
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Radioimmunoassay (RIA)
    Specificité
    Detects ~50-60 kDa. Recognizes both phosphorylated and non-phosphorylated forms.
     Réactivité croisée
    Boeuf (Vache), Humain, Souris, Rat
    Purification
    Protein G Purified
    Immunogène
    Partially purified rat CaMKII
    Clone
    6G9
    Isotype
    IgG1
    Top Product
    Discover our top product CAMK2G Anticorps primaire
  • Indications d'application
    • WB (1:10000)
    • IHC (1:2000)
    • ICC/IF (1:50)
    • optimal dilutions for assays should be determined by the user.
    Commentaires

    0.1 μg/ml was sufficient for detection of CamKII in 20 μg rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Conjugated antibodies should be stored at 4°C
  • Antigène
    CAMKII gamma (CAMK2G) (Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G))
    Autre désignation
    CaMKII (CAMK2G Produits)
    Synonymes
    anticorps CAMK, anticorps CAMK-II, anticorps CAMKG, anticorps 5930429P18Rik, anticorps Camkg, anticorps calcium/calmodulin dependent protein kinase II gamma, anticorps calcium/calmodulin-dependent protein kinase II gamma, anticorps CAMK2G, anticorps Camk2g
    Sujet
    CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).
    ID gène
    12322
    NCBI Accession
    NP_033922
    UniProt
    P11798
    Pathways
    Signalisation WNT, Interferon-gamma Pathway, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of long-term Neuronal Synaptic Plasticity
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