RAS anticorps (AA 31-43) (FITC)
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- Antigène Tous les produits RAS
- RAS
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Épitope
- AA 31-43
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp RAS est conjugé à/à la FITC
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificité
- Detects ~21 kDa.
- Réactivité croisée
- Boeuf (Vache), Humain, Souris, Rat
- Purification
- Protein G Purified
- Immunogène
- Synthetic peptide corresponding to amino acids 31-43 of human RAS. (The immunogen peptide is from the effector binding loop (l2) of the H, K and N-Ras (AA31-43). This sequence is identical yeast, slime mould, fungi, Xenopus, rat, mouse and chicken over these residues.)
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- Indications d'application
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- WB (1:1000)
- IHC (1:100)
- ICC/IF (1:200)
- optimal dilutions for assays should be determined by the user.
- Commentaires
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1 μg/ml of ABIN2485498 was sufficient for detection of Ras in 20 μg of Hela cell lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
- Stockage commentaire
- Conjugated antibodies should be stored at 4°C
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- Antigène
- RAS
- Abstract
- RAS Produits
- Synonymes
- anticorps asr, anticorps resistance to audiogenic seizures, anticorps ras
- Sujet
- The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTPbound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G-protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, in 30 % of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6).
- ID gène
- 18176
- NCBI Accession
- NP_035067
- UniProt
- B2KGV5
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