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RAS anticorps (AA 31-43) (FITC)

Reactivité: Humain WB, IHC, IF, ICC Hôte: Lapin Polyclonal FITC
N° du produit ABIN2485498
  • Antigène Tous les produits RAS
    RAS
    Épitope
    • 10
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 31-43
    Reactivité
    • 39
    • 20
    • 19
    • 9
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 37
    • 1
    • 1
    Lapin
    Clonalité
    • 28
    • 11
    Polyclonal
    Conjugué
    • 16
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp RAS est conjugé à/à la FITC
    Application
    • 25
    • 12
    • 12
    • 11
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificité
    Detects ~21 kDa.
     Réactivité croisée
    Boeuf (Vache), Humain, Souris, Rat
    Purification
    Protein G Purified
    Immunogène
    Synthetic peptide corresponding to amino acids 31-43 of human RAS. (The immunogen peptide is from the effector binding loop (l2) of the H, K and N-Ras (AA31-43). This sequence is identical yeast, slime mould, fungi, Xenopus, rat, mouse and chicken over these residues.)
  • Indications d'application
    • WB (1:1000)
    • IHC (1:100)
    • ICC/IF (1:200)
    • optimal dilutions for assays should be determined by the user.
    Commentaires

    1 μg/ml of ABIN2485498 was sufficient for detection of Ras in 20 μg of Hela cell lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Conjugated antibodies should be stored at 4°C
  • Antigène
    RAS
    Abstract
    RAS Produits
    Synonymes
    anticorps asr, anticorps resistance to audiogenic seizures, anticorps ras
    Sujet
    The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTPbound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G-protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, in 30 % of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6).
    ID gène
    18176
    NCBI Accession
    NP_035067
    UniProt
    B2KGV5
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