RAS (AA 31-43) anticorps (HRP)

Détails pour le produit réf. ABIN2485499, Fournisseur: Connectez-vous pour afficher
Antigène
  • asr
  • resistance to audiogenic seizures
  • ras
Épitope
AA 31-43
36
4
3
2
2
1
1
Reactivité
Boeuf (Vache), Humain, Souris, Rat (Rattus)
66
44
44
38
4
Hôte
Lapin
49
16
3
Clonalité
Polyclonal
Conjugué
HRP
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
65
38
18
18
18
5
4
3
2
Options
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N° du produit (Fournisseur)
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Immunogène Synthetic peptide corresponding to amino acids 31-43 of human RAS. (The immunogen peptide is from the effector binding loop (l2) of the H, K and N-Ras (AA31-43). This sequence is identical yeast, slime mould, fungi, Xenopus, rat, mouse and chicken over these residues.)
Specificité Detects ~21 kDa.
Purification Protein G Purified
Antigène
Sujet The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTPbound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G-protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, in 30 % of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6).
ID gène 18176
NCBI Accession NP_035067
UniProt B2KGV5
Indications d'application
  • WB (1:1000)
  • IHC (1:100)
  • ICC/IF (1:200)
  • optimal dilutions for assays should be determined by the user.
Commentaires

1 μg/ml of SPC-173 was sufficient for detection of Ras in 20 μg of Hela cell lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock -20 °C
Images (Fournisseur)
Immunofluorescence (fixed cells) (IF/ICC) image for anti-RAS (AA 31-43) antibody (HRP) (ABIN2485499) Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RAS Polyclonal Anti...
Immunohistochemistry (IHC) image for anti-RAS (AA 31-43) antibody (HRP) (ABIN2485499) Immunohistochemistry analysis using Rabbit Anti-RAS Polyclonal Antibody . Tissue: bac...
Western Blotting (WB) image for anti-RAS (AA 31-43) antibody (HRP) (ABIN2485499) Western blot analysis of Mouse Brain Cortex cell lysates showing detection of ~21 kDa...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-RAS (AA 31-43) antibody (HRP) (ABIN2485499) Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RAS Polyclonal Anti...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-RAS (AA 31-43) antibody (HRP) (ABIN2485499) Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RAS Polyclonal Anti...
Background publications Ma, Karplus: "Ligand-induced conformational changes in ras p21: a normal mode and energy minimization analysis." dans: Journal of molecular biology, Vol. 274, Issue 1, pp. 114-31, 1998 (PubMed).

Boguski, McCormick: "Proteins regulating Ras and its relatives." dans: Nature, Vol. 366, Issue 6456, pp. 643-54, 1994 (PubMed).

Avruch, Zhang, Kyriakis: "Raf meets Ras: completing the framework of a signal transduction pathway." dans: Trends in biochemical sciences, Vol. 19, Issue 7, pp. 279-83, 1994 (PubMed).

Buday, Downward: "Epidermal growth factor regulates p21ras through the formation of a complex of receptor, Grb2 adapter protein, and Sos nucleotide exchange factor." dans: Cell, Vol. 73, Issue 3, pp. 611-20, 1993 (PubMed).

Huang, Marshall, Hancock: "Plasma membrane-targeted ras GTPase-activating protein is a potent suppressor of p21ras function." dans: Molecular and cellular biology, Vol. 13, Issue 4, pp. 2420-31, 1993 (PubMed).

Bos: "ras oncogenes in human cancer: a review." dans: Cancer research, Vol. 49, Issue 17, pp. 4682-9, 1989 (PubMed).

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