PUMA anticorps (C-Term) (Alkaline Phosphatase (AP))
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- Antigène Voir toutes PUMA (BBC3) Anticorps
- PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
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Épitope
- C-Term
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp PUMA est conjugé à/à la Alkaline Phosphatase (AP)
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Application
- Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
- Specificité
- Detects ~23 kDa. Detects ~16 kDa bands sometimes, possibly corresponding to PUMAβ.
- Réactivité croisée
- Poulet, Humain, Souris
- Purification
- Protein A Purified
- Immunogène
- C-terminal amino acids of human PUMA
- Top Product
- Discover our top product BBC3 Anticorps primaire
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- Indications d'application
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- WB (1:1000)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
- Commentaires
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1 μg/ml of ABIN2485952 was sufficient for detection of PUMA in 20 μg of human K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 0.02 % sodium azide, Storage buffer may change when conjugated
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
- Stockage commentaire
- Conjugated antibodies should be stored at 4°C
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- Antigène
- PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
- Autre désignation
- PUMA (BBC3 Produits)
- Synonymes
- anticorps BBC3, anticorps Puma, anticorps JFY-1, anticorps JFY1, anticorps PUMA, anticorps PUMA/JFY1, anticorps BCL2 binding component 3, anticorps Bcl-2 binding component 3, anticorps BBC3, anticorps Bbc3, anticorps bbc3
- Sujet
- Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 up-regulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
- ID gène
- 27113
- NCBI Accession
- NP_001120712
- UniProt
- Q9BXH1
- Pathways
- Signalisation p53, Positive Regulation of Endopeptidase Activity
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