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ERK1 anticorps (Alkaline Phosphatase (AP))

MAPK3 Reactivité: Rat WB, IHC, IF, FACS, ICC Hôte: Lapin Polyclonal Alkaline Phosphatase (AP)
N° du produit ABIN2486918
  • Antigène Voir toutes ERK1 (MAPK3) Anticorps
    ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))
    Reactivité
    • 255
    • 177
    • 147
    • 46
    • 20
    • 19
    • 18
    • 13
    • 12
    • 11
    • 11
    • 7
    • 7
    • 6
    • 5
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    Rat
    Hôte
    • 262
    • 29
    • 4
    • 1
    • 1
    Lapin
    Clonalité
    • 238
    • 59
    Polyclonal
    Conjugué
    • 122
    • 25
    • 22
    • 20
    • 13
    • 11
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    Cet anticorp ERK1 est conjugé à/à la Alkaline Phosphatase (AP)
    Application
    • 249
    • 104
    • 86
    • 68
    • 65
    • 46
    • 42
    • 39
    • 36
    • 24
    • 18
    • 12
    • 12
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
    Specificité
    Detects ~44kda (ERK1) and ~42 kDa (ERK2).
     Réactivité croisée
    Poulet, Boeuf (Vache), Drosophila melanogaster, Humain, Souris, Rat, Mouton, Xenopus laevis
    Purification
    Peptide Affinity Purified
    Immunogène
    A 35 residue synthetic peptide, corresponding to Erk1 MAP kinase with the CGG spacer group added and the peptide coupled to KLH.
    Top Product
    Discover our top product MAPK3 Anticorps primaire
  • Indications d'application
    • WB (1:1000)
    • IHC (1:100)
    • ICC/IF (1:100)
    • FCM (1:100)
    • optimal dilutions for assays should be determined by the user.
    Commentaires

    A 1:1000 dilution of ABIN2486918 was sufficient for detection of ERK1/2 in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Conjugated antibodies should be stored at 4°C
  • Antigène
    ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))
    Autre désignation
    ERK1 (MAPK3 Produits)
    Synonymes
    anticorps ERK-1, anticorps ERK1, anticorps ERT2, anticorps HS44KDAP, anticorps HUMKER1A, anticorps P44ERK1, anticorps P44MAPK, anticorps PRKM3, anticorps p44-ERK1, anticorps p44-MAPK, anticorps Erk-1, anticorps Erk1, anticorps Ert2, anticorps Esrk1, anticorps Mnk1, anticorps Mtap2k, anticorps Prkm3, anticorps p44, anticorps p44erk1, anticorps p44mapk, anticorps ERK3, anticorps ERK6, anticorps P38GAMMA, anticorps PRKM12, anticorps SAPK-3, anticorps SAPK3, anticorps fi06b09, anticorps wu:fi06b09, anticorps zERK1, anticorps Tb08.10J17.940, anticorps MAPK1, anticorps MNK1, anticorps AW123708, anticorps Erk6, anticorps P38gamma, anticorps Prkm12, anticorps Sapk3, anticorps ATMAPK3, anticorps ATMPK3, anticorps T6D9.4, anticorps mitogen-activated protein kinase 3, anticorps mitogen-activated protein kinase 3, anticorps mitogen-activated protein kinase 12, anticorps mitogen activated protein kinase 3, anticorps mitogen-activated serine/threonine-protein kinase, anticorps MAPK3, anticorps Mapk3, anticorps MAPK12, anticorps mapk3, anticorps Tc00.1047053509475.10, anticorps Tb927.8.3550, anticorps Mapk12, anticorps CEK1, anticorps MPK3
    Sujet
    The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase (1). They are both located in the cytosol and mitochondria (2). While the role of cytosol ERK1/2 is well studied and involved in multiple cellular functions (2), the role of mitochondrial ERK1/2 remains poorly understood. Both ERK 1 and 2 are activated by MEK1 or MEK2, by dual phosphorylation of a threonine and tyrosine residue in the activation loop (TEY motif) (1, 3). Either phosphorylation alone can induce an electrophoretic mobility shift, but both are required for activation of the kinase. This dual phosphorylation is efficiently detected by phosphorylation state-specific antibody directed to the pTEpY motif. Once activated, MAP kinases phosphorylate a broad spectrum of substrates, including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases (4). ERK1/2 activation is generally thought to confer a survival advantage to cells (5), however there is increasing evidence that suggests that the activation of ERK1/2 also contributes to cell death under certain conditions (5). ERK1/2 also is activated in neuronal and renal epithelial cells upon exposure to oxidative stress and toxicants or deprivation of growth factors, and inhibition of the ERK pathway blocks apoptosis (5).
    ID gène
    50689
    NCBI Accession
    NP_059043
    UniProt
    P21708
    Pathways
    Signalisation MAPK, Signalisation RTK, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins
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