Expected / apparent Molecular Weight of the Antigene: 70 kDa Detection limit is 4 pg, 11 fmol, linear range of logit/log plot is 0.02-6 pmol,midrange (B/ B o =50 %) 112 pg, 300 fmol.
Purification
serum
Immunogène
KLH-conjugated synthetic peptide conserved in known higher plant HSC70 proteins including three isoforms of Arabidopsis thaliana HSC70-1 (NP_ 001119156.1), HSC70-2 (NP_195869.1) and HSC70-3 (NP_187555.1) as well as heat shock inducible Hsp70 of Arabidopsis thaliana At3g12580/T2E22_110 and At3g16030 and At3g12580/T2E22_110
Recommended Dilution: 1 : 3000 with standard ECL on 5 µg of protein per well (WB), 2-3 µl/protein extractof concentration 3-5 mg/mL.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 150 µL of sterile water.
Conseil sur la manipulation
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Stock
-20 °C
Norhayati, Hazlina, Asrenee, Emilin: "Magnitude and risk factors for postpartum symptoms: a literature review." dans: Journal of affective disorders, Vol. 175, pp. 34-52, (2015) (PubMed).
Muralidharan, Oksman, Pal, Lindquist, Goldberg: "Plasmodium falciparum heat shock protein 110 stabilizes the asparagine repeat-rich parasite proteome during malarial fevers." dans: Nature communications, Vol. 3, pp. 1310, (2012) (PubMed).
Alvarez, del Hoyo, García-Breijo, Reig-Armiñana, del Campo, Guéra, Barreno, Casano: "Different strategies to achieve Pb-tolerance by the two Trebouxia algae coexisting in the lichen Ramalina farinacea." dans: Journal of plant physiology, Vol. 169, Issue 18, pp. 1797-806, (2012) (PubMed).
Janicka-Russak, Kaba?a, Burzynski: "Different effect of cadmium and copper on H+-ATPase activity in plasma membrane vesicles from Cucumis sativus roots." dans: Journal of experimental botany, Vol. 63, Issue 11, pp. 4133-42, (2012) (PubMed).
anticorps APG-2, anticorps HS24/P52, anticorps HSPH2, anticorps RY, anticorps hsp70, anticorps hsp70RY, anticorps CG31354, anticorps HSP70, anticorps Hsp70Bb, anticorps hsp70B, anticorps hsp70Bb-prime, anticorps DmelCG5834, anticorps CG5834, anticorps HSPA1, anticorps HSP70B', anticorps HSPA6, anticorps ARABIDOPSIS HEAT SHOCK PROTEIN 70, anticorps ATHSP70, anticorps heat shock protein 70, anticorps LOC100305036, anticorps hsc70, anticorps Hsp70, anticorps Hsp70-1, anticorps Hsp70.1, anticorps hsp68, anticorps Hsp110, anticorps irp94, anticorps HSP70-2, anticorps HSPA1B, anticorps HSPA2, anticorps hsp70-5, anticorps HSP70-1, anticorps HSP70.1, anticorps HSP70.2, anticorps heat shock protein family A (Hsp70) member 4, anticorps CG5834 gene product from transcript CG5834-RA, anticorps heat shock protein 70, anticorps heat shock protein family A (Hsp70) member 6, anticorps heat shock 70kDa protein 2, anticorps heat shock 70 kD protein cognate, anticorps Hsp70 family chaperone, anticorps Heat shock protein 70, anticorps Heat shock protein 70, putative, anticorps heat shock protein 1B, anticorps heat shock protein family A member 4, anticorps heat shock 70kDa protein 1A, anticorps heat shock protein 1, anticorps Heat Shock Protein, anticorps heat shock cognate 70-kd protein, anticorps Heat shock 70 kDa protein 1A, anticorps HSPA4, anticorps Hsp70Bbb, anticorps HSP70, anticorps HSPA6, anticorps HSPA2, anticorps PCC7424_2419, anticorps Isop_1041, anticorps CGB_C3390W, anticorps Bacsa_1698, anticorps dnaK-B, anticorps LOC100305036, anticorps Hspa1b, anticorps Hspa4, anticorps HSPA1A, anticorps hsp1, anticorps hsp-70, anticorps hsp70, anticorps LOC108348108
Sujet
AGI Code: At3g12580 Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70), is a highly conserved protein and a member of the family of molecular chaperones.