DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L) anticorps

Détails pour le produit réf. ABIN251529
Antigène
  • NV11307
  • DOT1L
  • DOT1
  • KMT4
  • A630076O07
  • AW907654
  • Dot1
  • mDot1
  • histone-lysine N-methyltransferase, H3 lysine-79 specific
  • DOT1 like histone lysine methyltransferase
  • histone methyltransferase
  • DOT1-like, histone H3 methyltransferase (S. cerevisiae)
  • LOC100116648
  • DOT1L
  • DOT1
  • Dot1l
Reactivité
Humain, Souris
82
23
19
Hôte
Lapin
57
30
Clonalité
Polyclonal
Conjugué
Inconjugué
6
4
3
2
2
2
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
53
28
25
8
5
4
1
Options
Immunogène The immunogen recognized by this antibody maps to a region between residue1000 and 1050 of human DOT1-Like using the numbering given in entry NP_115871.1 (GeneID 84444).
Purification Immunogen affinity purified
Antigène
Autre désignation DOT1L (DOT1L Antibody Extrait)
Sujet Gene Symbol: DOT1L
Poids moléculaire Theoretical MW: 185 kDa
ID gène 84444
UniProt Q8TEK3
Indications d'application Western Blot 1:2000-1:10000, Chromatin Immunoprecipitation 1:10-1:500, Immunohistochemistry 1:200-1:1000, Immunocytochemistry/Immunofluorescence 1:50-1:500, Immunoprecipitation 2-5 μg/mg lysate, Immunohistochemistry-Paraffin 1:200-1:1000For IHC: Epitope retrieval with Tris-EDTA pH 9.0 is recommended. Chromatin Immunoprecipitation was reported in scientific literature. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Commentaires

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer TBS and 0.1 % BSA
Buffer contains: 0.09 % Sodium Azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Do not freeze.
Stock 4 °C
Stockage commentaire Store at 4°C. Do not freeze.
Images (Fournisseur)
Western Blotting (WB) image for anti-DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L) antibody (ABIN251529) Western Blot and Immunoprecipitation: DOT1L Antibody [ABIN251529] - Detection of Huma...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L) antibody (ABIN251529) Immunohistochemistry-Paraffin: DOT1L Antibody [ABIN2515295] - FFPE section of human c...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L) antibody (ABIN251529) Immunohistochemistry-Paraffin: DOT1L Antibody [ABIN2515295] - Human lung carcinoma.
 image for anti-DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L) antibody (ABIN251529) Chromatin Immunoprecipitation (ChIP): DOT1L Antibody - Chromatin from KOPN-8 cell li...
Produit citée dans: Szablewski, Bret, Kassambara, Devin, Cartron, Costes-Martineau, Moreaux: "An epigenetic regulator-related score (EpiScore) predicts survival in patients with diffuse large B cell lymphoma and identifies patients who may benefit from epigenetic therapy." dans: Oncotarget, Vol. 9, Issue 27, pp. 19079-19099, 2018 (PubMed).

Gilan, Lam, Becher, Lugo, Cannizzaro, Joberty, Ward, Wiese, Fong, Ftouni, Tyler, Stanley, MacPherson, Weng, Chan, Ghisi, Smil, Carpenter, Brown, Garton, Blewitt, Bannister, Kouzarides, Huntly et al.: "Functional interdependence of BRD4 and DOT1L in MLL leukemia. ..." dans: Nature structural & molecular biology, Vol. 23, Issue 7, pp. 673-81, 2016 (PubMed).

Oksenych, Zhovmer, Ziani, Mari, Eberova, Nardo, Stefanini, Giglia-Mari, Egly, Coin: "Histone methyltransferase DOT1L drives recovery of gene expression after a genotoxic attack." dans: PLoS genetics, Vol. 9, Issue 7, pp. e1003611, 2013 (PubMed). (Échantillon (espèces): Mouse (Murine)). Détails: Western Blotting

Shah, Henriksen: "A novel disrupter of telomere silencing 1-like (DOT1L) interaction is required for signal transducer and activator of transcription 1 (STAT1)-activated gene expression." dans: The Journal of biological chemistry, Vol. 286, Issue 48, pp. 41195-204, 2011 (PubMed).

Jacinto, Ballestar, Esteller: "Impaired recruitment of the histone methyltransferase DOT1L contributes to the incomplete reactivation of tumor suppressor genes upon DNA demethylation." dans: Oncogene, Vol. 28, Issue 47, pp. 4212-24, 2009 (PubMed). (Échantillon (espèces): Human). Détails: Chromatin Immunoprecipitation

Steger, Lefterova, Ying, Stonestrom, Schupp, Zhuo, Vakoc, Kim, Chen, Lazar, Blobel, Vakoc: "DOT1L/KMT4 recruitment and H3K79 methylation are ubiquitously coupled with gene transcription in mammalian cells." dans: Molecular and cellular biology, Vol. 28, Issue 8, pp. 2825-39, 2008 (PubMed).

Avez-vous cherché autre chose?