PIK3C3 anticorps (Phosphoinositide-3-Kinase, Class 3) (AA 700-850)

Details for Product anti-PIK3C3 Antibody No. ABIN259946, Fournisseur: Connectez-vous pour afficher
Antigène
  • im:7147158
  • wu:fc13g06
  • wu:fz51e12
  • zgc:112016
  • zgc:113009
  • VPS34
  • hVps34
  • 5330434F23Rik
  • Vps34
  • phosphatidylinositol 3-kinase catalytic subunit type 3
  • phosphatidylinositol 3-kinase catalytic subunit type 3 L homeolog
  • phosphatidylinositol 3-kinase, catalytic subunit type 3
  • PIK3C3
  • pik3c3.L
  • pik3c3
  • Pik3c3
Épitope
AA 700-850, Internal Region
22
22
22
15
15
10
8
8
7
7
7
7
7
7
7
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivité
Humain, Souris, Porc, Rat (Rattus)
166
71
61
2
Hôte
Lapin
164
2
Clonalité
Polyclonal
Conjugué
Cet anticorp PIK3C3 est non-conjugé
15
14
14
11
11
11
3
3
3
3
3
3
3
3
3
3
3
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Simple Western (SimWes), Western Blotting (WB)
100
88
71
39
22
17
15
15
10
2
1
1
1
1
Options
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N° du produit (Fournisseur)
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Immunogène Synthetic peptide made to an internal portion of the human VPS34 protein (within residues 700-850). [Swiss-Prot# Q8NEB9]
Purification Immunogen affinity purified
Autre désignation VPS34 (PIK3C3 Antibody Extrait)
Sujet Gene Symbol: PIK3C3
Poids moléculaire Theoretical MW: 100 kDa
ID gène 5289
UniProt Q8NEB9
Pathways AMPK Signaling, Activation of Innate immune Response, Inositol Metabolic Process, Toll-Like Receptors Cascades, Autophagy
Indications d'application Western Blot 1:1000, Simple Western 1:20, Immunocytochemistry/Immunofluorescence 1:100This VPS34 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen approx. 100 kDa. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Commentaires

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocole Protocol specific for VPS34 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-VPS34 primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer PBS, 0.1 % BSA, and 50 % Glycerol
Buffer contains: 0.05 % Sodium Azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Avoid freeze-thaw cycles
Stock 4 °C,-20 °C
Stockage commentaire Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Images (Fournisseur)
Western Blotting (WB) image for anti-Phosphoinositide-3-Kinase, Class 3 (PIK3C3) (AA 700-850), (Internal Region) antibody (ABIN259946) Detection of VPS34 in HepG2 whole cell lysates using ABIN259946.
Simple Western (SimWes) image for anti-Phosphoinositide-3-Kinase, Class 3 (PIK3C3) (AA 700-850), (Internal Region) antibody (ABIN259946) Simple Western: VPS34 Antibody [ABIN2599460] - Simple Western lane view shows a speci...
Immunofluorescence (IF) image for anti-Phosphoinositide-3-Kinase, Class 3 (PIK3C3) (AA 700-850), (Internal Region) antibody (ABIN259946) Immunocytochemistry/Immunofluorescence: VPS34 Antibody [ABIN2599460] - VPS34 antibody...
Produit citée dans: Brigger, Schläfli, Garattini, Tschan: "Activation of RARα induces autophagy in SKBR3 breast cancer cells and depletion of key autophagy genes enhances ATRA toxicity." dans: Cell death & disease, Vol. 6, pp. e1861, 2015 (PubMed). (Échantillon (espèces): Human). Détails: Western Blotting

Shi, Bai, Zhang, Yiu, Yin, Han, Liu, Li, Fu, Ye: "Saxifragifolin D induces the interplay between apoptosis and autophagy in breast cancer cells through ROS-dependent endoplasmic reticulum stress." dans: Biochemical pharmacology, Vol. 85, Issue 7, pp. 913-26, 2013 (PubMed). (Échantillon (espèces): Human). Détails: Western Blotting

Huang, Hou, Chen, Zhao, Yan, Zhang, Yang, Kogan, Chen: "PML-RARα enhances constitutive autophagic activity through inhibiting the Akt/mTOR pathway." dans: Autophagy, Vol. 7, Issue 10, pp. 1132-44, 2011 (PubMed). (Échantillon (espèces): Human). Détails: Western Blotting

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