His Tag anticorps

Détails pour le produit réf. ABIN2688142
Antigène
Reactivité
Tag
438
24
9
2
1
Hôte
Souris
288
164
23
21
8
8
3
2
Clonalité (Clone)
Monoclonal ()
Conjugué
Inconjugué
45
37
26
13
10
6
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Dot Blot (DB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
445
259
149
63
56
34
33
14
14
11
8
8
7
7
7
6
6
5
4
4
1
1
1
1
1
1
1
1
Options
Immunogène 6x His synthetic peptide.
Séquence HHHHHH
Clone HIS-H8
Isotype IgG2b
Specificité His-tag fusion protein
Purification IgG fraction
Antigène
Classe de substances Tag
Sujet The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Indications d'application ICC/IHC/IF/IP 1/200. WB 1/1000. Other applications are optimized by end users.
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer PBS, pH 7.4 with 0.05 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C,-20 °C
Produit citée dans: Wyroba, Kwaśniak, Miller, Kobyłecki, Osińska: "Site-directed mutagenesis, in vivo electroporation and mass spectrometry in search for determinants of the subcellular targeting of Rab7b paralogue in the model eukaryote Paramecium octaurelia." dans: European journal of histochemistry : EJH, Vol. 60, Issue 2, pp. 2612, 2016 (PubMed). Méthode utilisée: Confocal Microscopy (CM)

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