Cardiac Troponin T2 anticorps (PE)

N° du produit ABIN2688864

Détail du produit anti-Cardiac Troponin T2 anticorps (PE)

Antigène: Cardiac Troponin T2 (cTnT) (Cardiac Troponin T (cTnT))

Reactivité: Humain, Souris, Rat, Porc, Lapin, Chien, Poulet, Cobaye

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cardiac Troponin T2 est conjugé à/à la PE

Application: Intracellular Staining (ICS)

Marque: BD Pharmingen™

Attributs du produit: The 13-11 monoclonal antibody specifically recognizes Troponin T type 2 (cardiac), encoded by the gene TNNT2. Troponin T is the tropomyosin-binding subunit of the troponin complex, which also encompasses troponin C and troponin I. This complex regulates muscle contraction in skeletal and cardiac muscle in response to alterations in calcium levels. Troponin T type 2 is solely found in the heart, and genetic alterations in the TNNT2 gene are associated to a series of heart disorders in humans, including hypertrophic cardiomyopathy, dilated cardiomyopathy and left ventricular noncompaction. Cardiac Troponin T can be used as a marker for the identification of cardiomyocytes derived from pluripotent stem cells. Flow cytometric analysis of Cardiac Troponin T in human cardiomyocytes differentiated in vitro. Human embryonic stem cell-derived cardiomyocytes (Evans lab, UCSD) were disassociated and fixed in BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm/Wash Buffer I (Cat. No. 557885). The cells were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680, dashed line) or PE Mouse Anti-Cardiac Troponin T (solid line). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD LSR Fortessa™ flow cytometry system. Flow cytometric analysis of Cardiac Troponin T in mouse cardiomyocytes differentiated in vitro. The C2C12 mouse myoblast cell line (ATCC CRL-1772) was cultured for 5 days in low-serum conditions for induction of cell differentiation, fixed with BD Cytofix™ Fixation Buffer, and permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723). The cells were stained with either PE Mouse IgG1, κ Isotype Control (dashed line) or PE Mouse Anti-Cardiac Troponin T (solid line). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD FACSCanto™ II flow cytometry system. 564767 Rev. 1 Page 1 of 2

BD Pharmingen™ PE Mouse Anti-Cardiac Troponin T - PE - Clone 13-11 - Isotype Mouse IgG1, κ - Reactivity Ms, Hu, Chick, Dog, G Pig, Pig, Rab, Rat - 50 Tests

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Rabbit cardiac troponin T Protein

Clone: 13

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 5 μL

Restrictions: For Research Use only

Stockage

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-Cardiac Troponin T2 (PE) (ABIN2688864)

Jáchymová, Muravská, Paleček, Kuchynka, Řeháková, Magage, Kr��l, Zima, Horký, Linhart: "Genetic variation screening of TNNT2 gene in a cohort of patients with hypertrophic and dilated cardiomyopathy." dans: Physiological research / Academia Scientiarum Bohemoslovaca, Vol. 61, Issue 2, pp. 169-75, (2012) (PubMed).

Lian, Hsiao, Wilson, Zhu, Hazeltine, Azarin, Raval, Zhang, Kamp, Palecek: "Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 27, pp. E1848-57, (2012) (PubMed).

Uosaki, Fukushima, Takeuchi, Matsuoka, Nakatsuji, Yamanaka, Yamashita: "Efficient and scalable purification of cardiomyocytes from human embryonic and induced pluripotent stem cells by VCAM1 surface expression." dans: PLoS ONE, Vol. 6, Issue 8, pp. e23657, (2011) (PubMed).

Zhang, Wilson, Soerens, Koonce, Yu, Palecek, Thomson, Kamp: "Functional cardiomyocytes derived from human induced pluripotent stem cells." dans: Circulation research, Vol. 104, Issue 4, pp. e30-41, (2009) (PubMed).

Mauritz, Schwanke, Reppel, Neef, Katsirntaki, Maier, Nguemo, Menke, Haustein, Hescheler, Hasenfuss, Martin: "Generation of functional murine cardiac myocytes from induced pluripotent stem cells." dans: Circulation, Vol. 118, Issue 5, pp. 507-17, (2008) (PubMed).

Thierfelder, Watkins, MacRae, Lamas, McKenna, Vosberg, Seidman, Seidman: "Alpha-tropomyosin and cardiac troponin T mutations cause familial hypertrophic cardiomyopathy: a disease of the sarcomere." dans: Cell, Vol. 77, Issue 5, pp. 701-12, (1994) (PubMed).

Malouf, McMahon, Oakeley, Anderson: "A cardiac troponin T epitope conserved across phyla." dans: The Journal of biological chemistry, Vol. 267, Issue 13, pp. 9269-74, (1992) (PubMed).

Détails sur Cardiac Troponin T2

Antigène: Cardiac Troponin T2 (cTnT) (Cardiac Troponin T (cTnT))

Autre désignation: Cardiac Troponin T (cTnT Produits)

Synonymes: anticorps CMH2, anticorps CMPD2, anticorps LVNC6, anticorps RCM3, anticorps TnTC, anticorps cTnT, anticorps Tnt, anticorps CTTG, anticorps Ctt, anticorps RATCTTG, anticorps Tnnt3, anticorps tnnt2, anticorps CT3, anticorps si:ch211-136g2.1, anticorps troponin T2, cardiac type, anticorps troponin T2, cardiac, anticorps troponin T type 2a (cardiac), anticorps troponin T2, cardiac type L homeolog, anticorps troponin T2e, cardiac, anticorps TNNT2, anticorps Tnnt2, anticorps tnnt2a, anticorps tnnt2.L, anticorps tnnt2e

Sujet: Synonyms: cTnT, Cardiac Muscle Troponin T, Troponin T type 2, TNNT2