CD11c anticorps (Biotin)

N° du produit ABIN2688921

Détail du produit anti-CD11c anticorps (Biotin)

Antigène: CD11c (ITGAX) (Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX))

Reactivité: Souris

Hôte: Hamster arménien

Clonalité: Monoclonal

Conjugué: Cet anticorp CD11c est conjugé à/à la Biotin

Application: Flow Cytometry (FACS)

Marque: BD Pharmingen™

Attributs du produit: The HL3 antibody reacts with the integrin αx chain of gp150, 95 (CD11c/CD18) which is expressed on dendritic cells and CD4- CD8+ intestinal intraepithelial lymphocytes (IEL) and is upregulated on IEL and lymph-node T cells following in vivo activation. CD11c is also found on human NK cells. Although its expression on mouse NK cells is not published, we have detected CD11c on mouse splenic NK cells. Cells of the monocyte/macrophage lineage have been reported to express low levels of CD11c. CD11c plays a role in binding of iC3b. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Expression of CD11c on spleen NK cells. C57BL/6 splenocytes were stained simultaneously with PE-conjugated anti-mouse NK-1.1 mAb PK136 (Cat. No. 557391/553165) and isotype control (left panel) or biotin-conjugated mAb HL3 (right panel), followed by Avidin-FITC (Cat. No. 554057). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Biotin Hamster Anti-Mouse CD11c - Biotin - Clone HL3 - Isotype Armenian Hamster IgG1, λ2 - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: C57BL/6 Mouse Intestinal Intraepithelial Lymphocytes

Clone: HL3

Isotype: IgG1 lambda

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CD11c (Biotin) (ABIN2688921)

Gao, Liu, Wen, Zhang, Durbin, Liu, Zheng: "Differentiation of monocytic cell clones into CD8 alpha+ dendritic cells (DC) suggests that monocytes can be direct precursors for both CD8 alpha+ and CD8 alpha- DC in the mouse." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 5927-35, (2003) (PubMed).

Pulendran, Lingappa, Kennedy, Smith, Teepe, Rudensky, Maliszewski, Maraskovsky: "Developmental pathways of dendritic cells in vivo: distinct function, phenotype, and localization of dendritic cell subsets in FLT3 ligand-treated mice." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 5, pp. 2222-31, (1997) (PubMed).

Maraskovsky, Brasel, Teepe, Roux, Lyman, Shortman, McKenna: "Dramatic increase in the numbers of functionally mature dendritic cells in Flt3 ligand-treated mice: multiple dendritic cell subpopulations identified." dans: The Journal of experimental medicine, Vol. 184, Issue 5, pp. 1953-62, (1996) (PubMed).

Huleatt, Lefrançois: "Antigen-driven induction of CD11c on intestinal intraepithelial lymphocytes and CD8+ T cells in vivo." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 11, pp. 5684-93, (1995) (PubMed).

Larson, Springer: "Structure and function of leukocyte integrins." dans: Immunological reviews, Vol. 114, pp. 181-217, (1990) (PubMed).

Détails sur CD11c

Antigène: CD11c (ITGAX) (Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX))

Autre désignation: CD11c (ITGAX Produits)

Synonymes: anticorps CD11C, anticorps SLEB6, anticorps RGD1561123, anticorps AI449405, anticorps Cd11c, anticorps Cr4, anticorps N418, anticorps integrin subunit alpha X, anticorps integrin alpha X, anticorps ITGAX, anticorps Itgax

Sujet: Synonyms: Integrin αx chain

Pathways: Système du Complément, Activated T Cell Proliferation, Integrin Complex