CD137 anticorps (Biotin)

N° du produit ABIN2688964

Détail du produit anti-CD137 anticorps (Biotin)

Antigène: CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD137 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS)

Marque: BD Pharmingen™

Attributs du produit: The 1AH2 clone (subclone of 53A2) has been reported to react with CD137, a member of the TNFR/NGFR superfamily. The expression of CD137 has been reported to be detectable from day 3 and peaks around day 6 after activation with ConA, PMA plus ionomycin, or immobilized anti-CD3e (Cat. No. 553058). Monomers, dimers, or tetramers of the 4-1BB antigen are expressed, upon activation, on the surface of splenic T lymphocytes, thymocytes, intestinal intraepithelial T lymphocytes (IEL), and some T cell lines and clones. While stimulating T cells by IL-2, IL-4, or anti-CD28 alone does not result in the expression of CD137, addition of IL-2, IL-4, anti-CD28, or syngeneic accessory cells to splenic T cells stimulated via TCR/CD3 can result in a high level of CD137 expression. CD137 has also been reported to be observed on IL-2 activated NK cells, but not on freshly isolated NK cells. It has been demonstrated that 4-1BB physically associates with p56 [lck]through a Cys-Arg-Cys-Pro binding site in its cytoplasmic domain, the same motif in the cytoplasmic tail of the CD4 and CD8a molecules is responsible for association with p56 [lck]. A signaling function for the CD137 Molecule in mouse T cells is indicated by reports in which cross-linking of CD137 with 1AH2 mAb resulted in enhanced proliferation of CD3e-activated splenic T cells and IEL and in enhanced cytolytic activity of IEL in response to immobilized anti-CD3e. In addition to extracellular matrix proteins which bind to CD137, a 97- kDa dimer of the TNF/NGF superfamily has been reported to be a ligand for 4-1BB (4-1BBL). This molecule has been detected on Con A-activated T cells, LPS-activated macrophages, and anti-μ-activated splenic B cells. Interaction between T and B cells through 4-1BB/4-1BBL is reported to play a role in antigen presentation, further supporting a costimulatory role for CD137 in the immune response of T lymphocytes. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Biotin Rat Anti-Mouse CD137 - Biotin - Clone 1AH2 - Isotype Rat IgG1, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Recombinant mouse 4-1BB

Clone: 1AH2

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CD137 (Biotin) (ABIN2688964)

Takeda, Oshima, Hayakawa, Akiba, Atsuta, Kobata, Kobayashi, Ito, Yagita, Okumura: "CD27-mediated activation of murine NK cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 4, pp. 1741-5, (2000) (PubMed).

Saoulli, Lee, Cannons, Yeh, Santana, Goldstein, Bangia, DeBenedette, Mak, Choi, Watts: "CD28-independent, TRAF2-dependent costimulation of resting T cells by 4-1BB ligand." dans: The Journal of experimental medicine, Vol. 187, Issue 11, pp. 1849-62, (1998) (PubMed).

DeBenedette, Shahinian, Mak, Watts: "Costimulation of CD28- T lymphocytes by 4-1BB ligand." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 2, pp. 551-9, (1997) (PubMed).

Hurtado, Kim, Kwon: "Signals through 4-1BB are costimulatory to previously activated splenic T cells and inhibit activation-induced cell death." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 6, pp. 2600-9, (1997) (PubMed).

DeBenedette, Chu, Pollok, Hurtado, Wade, Kwon, Watts: "Role of 4-1BB ligand in costimulation of T lymphocyte growth and its upregulation on M12 B lymphomas by cAMP." dans: The Journal of experimental medicine, Vol. 181, Issue 3, pp. 985-92, (1995) (PubMed).

Hurtado, Kim, Pollok, Lee, Kwon: "Potential role of 4-1BB in T cell activation. Comparison with the costimulatory molecule CD28." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 7, pp. 3360-7, (1995) (PubMed).

Pollok, Kim, Kwon: "Regulation of 4-1BB expression by cell-cell interactions and the cytokines, interleukin-2 and interleukin-4." dans: European journal of immunology, Vol. 25, Issue 2, pp. 488-94, (1995) (PubMed).

Zhou, Pollok, Kim, Kim, Kwon: "Functional analysis of T-cell antigen 4-1BB in activated intestinal intra-epithelial T lymphocytes." dans: Immunology letters, Vol. 41, Issue 2-3, pp. 177-84, (1995) (PubMed).

Kim, Pollok, Zhou, Shaw, Bohlen, Fraser, Kwon: "Novel T cell antigen 4-1BB associates with the protein tyrosine kinase p56lck1." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 3, pp. 1255-62, (1993) (PubMed).

Pollok, Kim, Zhou, Hurtado, Kim, Pickard, Kwon: "Inducible T cell antigen 4-1BB. Analysis of expression and function." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 3, pp. 771-81, (1993) (PubMed).

Chalupny, Peach, Hollenbaugh, Ledbetter, Farr, Aruffo: "T-cell activation molecule 4-1BB binds to extracellular matrix proteins." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 21, pp. 10360-4, (1992) (PubMed).

Détails sur CD137

Antigène: CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))

Autre désignation: CD137 (TNFRSF9 Produits)

Synonymes: anticorps TNFRSF9, anticorps zgc:136557, anticorps 4-1BB, anticorps CD137, anticorps CDw137, anticorps ILA, anticorps A930040I11Rik, anticorps AA408498, anticorps AI325004, anticorps Cd137, anticorps Ly63, anticorps tumor necrosis factor receptor superfamily member 9, anticorps TNF receptor superfamily member 9, anticorps tumor necrosis factor receptor superfamily, member 9a, anticorps tumor necrosis factor receptor superfamily, member 9, anticorps TNFRSF9, anticorps tnfrsf9a, anticorps Tnfrsf9

Sujet: Synonyms: 4-1BB, Ly-63

Pathways: Cancer Immune Checkpoints