IL-10RA anticorps

N° du produit ABIN2689090

Détail du produit anti-IL-10RA anticorps

Antigène: IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp IL-10RA est non-conjugé

Application: Flow Cytometry (FACS)

Marque: BD Pharmingen™

Attributs du produit: The 1B1.3a antibody reacts with the extracellular region of mouse CD210 which is also known as the mouse IL-10 receptor (mIL-10R), it does not recognize the human IL-10R. The IL-10R is expressed by a variety of mouse cell types and cell lines including thymocytes, T cells, B cells, and monocytes. 1B1.3a is a neutralizing antibody and reportedly blocks the binding of human IL-10, which cross-reacts with the mouse IL-10R. The immunogen used for the generation of the 1B1.3a hybridoma was purified recombinant ligand-binding domain of mIL-10R. Mouse B220+ splenocytes were stained with purified 1B1.3a (0.25 μg, Cat. No. 559912) followed by biotinylated polyclonal goat anti-rat Ig (0.25 μg, Cat. No. 554014) and Streptavidin-PE (0.015 μg, Cat. No. 554061) in a three layer staining protocol to amplify immunofluorescent signals (left panel). In addition, cells in buffer containing sodium azide were pre-incubated on ice with recombinant mouse IL-10 (0.25 μg, Cat. No. 554585) prior to staining with 1B1.3a to determine the blocking effect of bound IL-10 protein on staining with 1B1.3a (right panel). Staining with the purified 1B1.3a antibody (filled histograms) is compared to the staining obtained with the secondary antibody alone (open histograms). Histograms in both panels are gated on the lymphocyte population as defined by light scattering characteristics and were also gated on the cell subset stained with FITC-conjugated rat-anti-mouse B220 (0.06 μg, Cat. No. 553088).

BD Pharmingen™ Purified Rat Anti-Mouse CD210 - Purified - Clone 1B1.3a - Isotype Rat IgG1, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Purified recombinant ligand-binding domain of mIL-10R

Clone: 1B1-3a

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-IL-10RA (ABIN2689090)

OFarrell, Liu, Moore, Mui: "IL-10 inhibits macrophage activation and proliferation by distinct signaling mechanisms: evidence for Stat3-dependent and -independent pathways." dans: The EMBO journal, Vol. 17, Issue 4, pp. 1006-18, (1998) (PubMed).

Détails sur IL-10RA

Antigène: IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))

Autre désignation: CD210 (IL10RA Produits)

Synonymes: anticorps IL10R1, anticorps IL10RA, anticorps CD210, anticorps CD210a, anticorps CDW210A, anticorps HIL-10R, anticorps IL-10R1, anticorps IL10R, anticorps AW553859, anticorps CDw210, anticorps CDw210a, anticorps Il10r, anticorps mIL-10R, anticorps interleukin 10 receptor subunit alpha, anticorps interleukin 10 receptor, alpha, anticorps IL10RA, anticorps Il10ra

Sujet: Synonyms: IL-10 Receptor

Pathways: Growth Factor Binding