E-cadherin anticorps

N° du produit ABIN2689191

Détail du produit anti-E-cadherin anticorps

Antigène: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp E-cadherin est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), BioImaging (BI)

Marque: BD Pharmingen™

Attributs du produit: The 67A4 monoclonal antibody recognizes the extracellular domain of human E-Cadherin (CD324). E-Cadherin is a 120- kDa transmembrane glycoprotein that is localized in the adherens junctions of epithelial cells. There it interacts with the cytoskeleton through the associated cytoplasmic catenin proteins. In addition to being a calcium-dependent adhesion molecule, E-Cadherin is also a critical regulator of epithelial junction formation. Its association with catenins is necessary for cell-to-cell adhesion. These E-Cadherin/catenin complexes associate with cortical actin bundles at both the zonula adherens and the lateral adhesion plaques. Tyrosine phosphorylation can disrupt these complexes, leading to changes in cell adhesion properties. E-Cadherin expression is often down-regulated in highly invasive, poorly differentiated carcinomas. Increased expression of E-Cadherin in these cells reduces their invasiveness. Thus, loss of expression or function of E-Cadherin appears to be an important step in tumorigenic progression. Pluripotent stem cells express E-Cadherin. Upon differentiation, an epithelial to mesenchymal transition results in the loss of E-cadherin expression and a gain in the expression of N-cadherin. Western blot analysis of CD324 (E-cadherin) expression in human breast adenocarcinoma and human embryonic stem (ES) cells. Cell Lysates from a human breast adenocarcinoma cell line MCF-7 (ATCC HTB-22, left blot) and H9 human ES Cells (WiCell, Madison, WI, right blot) were probed with Purified Mouse Anti-Human CD324 (E-Cadherin) monoclonal antibody at titrations of 0.125 (lanes 1), 0.063 (lanes 2), 0.032 (lanes 3) μg/mL. Proteins were detected using HRP Goat Anti-Mouse Ig (Cat. No. 554002). CD324 (E-cadherin) is identified as a band of ~120 kDa in MCF7 and Human ES cells. Immunoflourescent staining of CD324 (E-cadherin) on human embryonic stem (ES) cells. H9 human ES cells (WiCell, Madison, WI) passage 45 grown in mTESR™1 media (StemCell Technologies) on BD Matrigel™ hESC-qualified Matrix (Cat. No. 354277) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). Cells were stained with Purified Mouse Anti-Human CD324 (E-Cadherin) monoclonal antibody (pseudo-colored green) at 2.5 μg/mL. The second-step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies), and cell nuclei were stained with DAPI (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ software.

BD Pharmingen™ Purified Mouse Anti-Human CD324 (E-Cadherin) - Purified - Clone 67A4 - Isotype Mouse IgG1, κ - Reactivity Hu - 50 μg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Breast Tumor Cell Line

Clone: 67A4

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-E-cadherin (ABIN2689191)

DAmour, Agulnick, Eliazer, Kelly, Kroon, Baetge: "Efficient differentiation of human embryonic stem cells to definitive endoderm." dans: Nature biotechnology, Vol. 23, Issue 12, pp. 1534-41, (2005) (PubMed).

Cepek, Shaw, Parker, Russell, Morrow, Rimm, Brenner: "Adhesion between epithelial cells and T lymphocytes mediated by E-cadherin and the alpha E beta 7 integrin." dans: Nature, Vol. 372, Issue 6502, pp. 190-3, (1994) (PubMed).

Takeichi: "The cadherins: cell-cell adhesion molecules controlling animal morphogenesis." dans: Development (Cambridge, England), Vol. 102, Issue 4, pp. 639-55, (1988) (PubMed).

Détails sur E-cadherin

Antigène: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Autre désignation: CD324 E-Cadherin (CDH1 Produits)

Synonymes: anticorps Arc-1, anticorps CD324, anticorps CDHE, anticorps ECAD, anticorps LCAM, anticorps UVO, anticorps E-cadherin, anticorps cdh1, anticorps si:dz180o5.2, anticorps uvo, anticorps lcam, anticorps E-Cad, anticorps XBcad, anticorps l-cam, anticorps xcdh1, anticorps cdhc-A, anticorps xb-cad, anticorps XTCAD-1, anticorps c-cadherin, anticorps cadherin-1, anticorps uvomorulin, anticorps XB-cadherin, anticorps arc-1, anticorps cd324, anticorps cdhe, anticorps ecad, anticorps AA960649, anticorps E-cad, anticorps Ecad, anticorps L-CAM, anticorps Um, anticorps cadherin 1, anticorps si:busm1-180o5.2, anticorps cadherin 1, type 1, E-cadherin (epithelial), anticorps cadherin 1, type 1 S homeolog, anticorps CDH1, anticorps si:busm1-180o5.2, anticorps cdh1, anticorps Cdh1, anticorps cdh1.S

Sujet: Synonyms: E-cadherin

Poids moléculaire: ~120 kDa

Pathways: Signalisation WNT, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation