CD86 anticorps
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- Antigène Voir toutes CD86 Anticorps
- CD86
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Reactivité
- Souris
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Hôte
- Rat
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD86 est non-conjugé
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Application
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR)
- Marque
- BD Pharmingen™
- Attributs du produit
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The GL1 antibody has been reported to react with the B7-2 (CD86) costimulatory molecule expressed on a broad spectrum of leukocytes, including B lymphocytes, T lymphocytes, thioglycollate-induced peritoneal macrophages, dendritic cells and astrocytes. CD86 is expressed at low levels by freshly explanted peripheral B and T cells, and its expression is substantially increased by a variety of T cell- and B cell-specific stimuli with a peak expression after 18-42 hours of culture. In contrast to most naive CD4+ T cells, memory CD4+ T cells express B7-2, both at the mRNA and protein level. CD86, a ligand for CD28 and CD152 (CTLA-4), is one of the accessory molecules that plays an important role in T cell-B cell costimulatory interactions. It has been shown to be involved in immunoglobulin class-switching and triggering of mouse NK cell-mediated cytotoxicity. CD80 (B7-1) is an alternate ligand for CD28 and CD152 (CTLA-4). GL1 antibody reportedly blocks MLR and stimulation of T cells by natural antigen-presenting cells. In addition, a mixture of anti-B7-1 and anti B7-2 (GL1) mAbs reportedly inhibits the in vitro interaction of CTLA-4 with its ligand and the in vivo priming of cytotoxic T lymphocytes. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Upregulation of membrane CD86 (B7-2) on activated B lymphocytes. Freshly isolated (left panel) or 72-hour LPS-stimulated BALB/c splenocytes (right panel) were pretreated with Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. No. 553141/553142) and either unstained (shaded histograms) or stained with purified GL1 mAb (open histograms) followed by FITC-conjugated anti-rat IgG2a mAb RG7/1.30 (Cat. No. 553896, shaded and open histograms). Flow cytometry was performed on a BD FACScan™
BD Pharmingen™ Purified Rat Anti-Mouse CD86 - Purified - Clone GL1 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogène
- Mouse (CBA/Ca) LPS-activated splenic B Cells
- Clone
- GL1
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product CD86 Anticorps primaire
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- Indications d'application
- Flow Cytometry System. Resting lymphocytes (left panel) or activated blasts (right panel) were selected according to light-scatter profile.
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
- Stockage commentaire
- Store undiluted at 4°C.
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Transport of peptide-MHC class II complexes in developing dendritic cells." dans: Science (New York, N.Y.), Vol. 288, Issue 5465, pp. 522-7, (2000) (PubMed).
: "Triggering of murine NK cells by CD40 and CD86 (B7-2)." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 10, pp. 5910-6, (1999) (PubMed).
: "The role of B7 co-stimulation in activation and differentiation of CD4+ and CD8+ T cells." dans: Immunological reviews, Vol. 165, pp. 231-47, (1999) (PubMed).
: "Expression and co-stimulatory function of B7-2 on murine CD4+ T cells." dans: European journal of immunology, Vol. 28, Issue 3, pp. 865-73, (1998) (PubMed).
: "B7-1 and B7-2 have overlapping, critical roles in immunoglobulin class switching and germinal center formation." dans: Immunity, Vol. 6, Issue 3, pp. 303-13, (1997) (PubMed).
: "CD28/B7 costimulation regulates autoimmune diabetes induced with multiple low doses of streptozotocin." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 2, pp. 984-91, (1997) (PubMed).
: "Distinct costimulatory molecules are required for the induction of effector and memory cytotoxic T lymphocytes." dans: The Journal of experimental medicine, Vol. 185, Issue 2, pp. 251-62, (1997) (PubMed).
: "IFN-gamma-activated primary murine astrocytes express B7 costimulatory molecules and prime naive antigen-specific T cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 2, pp. 614-21, (1997) (PubMed).
: "New perspectives of CD28-B7-mediated T cell costimulation." dans: Immunity, Vol. 2, Issue 6, pp. 555-9, (1995) (PubMed).
: "Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function." dans: The Journal of experimental medicine, Vol. 180, Issue 2, pp. 631-40, (1994) (PubMed).
: "The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro." dans: The Journal of experimental medicine, Vol. 180, Issue 5, pp. 1849-60, (1994) (PubMed).
: "Regulation of immunostimulatory function and costimulatory molecule (B7-1 and B7-2) expression on murine dendritic cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 11, pp. 5208-19, (1994) (PubMed).
: "Expression and functional significance of an additional ligand for CTLA-4." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 23, pp. 11054-8, (1994) (PubMed).
: "Uncovering of functional alternative CTLA-4 counter-receptor in B7-deficient mice." dans: Science (New York, N.Y.), Vol. 262, Issue 5135, pp. 907-9, (1993) (PubMed).
: "Identification of an alternative CTLA-4 ligand costimulatory for T cell activation." dans: Science (New York, N.Y.), Vol. 262, Issue 5135, pp. 905-7, (1993) (PubMed).
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Transport of peptide-MHC class II complexes in developing dendritic cells." dans: Science (New York, N.Y.), Vol. 288, Issue 5465, pp. 522-7, (2000) (PubMed).
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- Antigène
- CD86
- Autre désignation
- CD86 (CD86 Produits)
- Synonymes
- anticorps B7-2, anticorps B7.2, anticorps B70, anticorps CD28LG2, anticorps LAB72, anticorps B7, anticorps CLS1, anticorps Cd28l2, anticorps ETC-1, anticorps Ly-58, anticorps Ly58, anticorps MB7, anticorps MB7-2, anticorps TS/A-2, anticorps CD86 molecule, anticorps CD86 antigen, anticorps integrin subunit alpha 2b, anticorps CD86, anticorps Cd86, anticorps Itga2b
- Sujet
- Synonyms: B7-2
- Pathways
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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