The BVD6-24G2 antibody reacts with mouse interleukin-4 (IL-4). The immunogen used to generate the BVD6-24G2 hybridoma was recombinant IL-4. This is a non-neutralizing antibody. This antibody is routinely tested by ELISA Detection. Other applications were tested during antibody development only or reported in the literature.
BD Pharmingen™ Biotin Rat Anti-Mouse IL-4 - Biotin - Clone BVD6-24G2 - Isotype Rat IgG1 - Reactivity Ms - 0.5 mg
Purification
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Stock
4 °C
Stockage commentaire
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Shirai, Sierra, Kelly, Klinman: "Individual cells simultaneously produce both IL-4 and IL-6 in vivo." dans: Cytokine, Vol. 6, Issue 3, pp. 329-36, (1994) (PubMed).
Bogen, Fogelman, Abbas: "Analysis of IL-2, IL-4, and IFN-gamma-producing cells in situ during immune responses to protein antigens." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 10, pp. 4197-205, (1993) (PubMed).
Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." dans: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).
Chatelain, Varkila, Coffman: "IL-4 induces a Th2 response in Leishmania major-infected mice." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 4, pp. 1182-7, (1992) (PubMed).