HARS1/Jo-1 anticorps

N° du produit ABIN272164

Détail du produit anti-HARS1/Jo-1 anticorps

Antigène: HARS1/Jo-1 (HARS1) (Histidyl-tRNA Synthetase (HARS1))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HARS1/Jo-1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: This antibody detects endogenous levels of HisRS protein. (region surrounding Glu492)

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse.

Purification: Affinity Chromatography using epitope-specific immunogen.

Information d'application

Indications d'application: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 15 mM Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur HARS1/Jo-1

Antigène: HARS1/Jo-1 (HARS1) (Histidyl-tRNA Synthetase (HARS1))

Autre désignation: Histidyl-tRNA Synthetase / HARS (HARS1 Produits)

Synonymes: anticorps HRS, anticorps USH3B, anticorps MMHRS, anticorps Dnd1, anticorps HARSL, anticorps histidyl-tRNA synthetase, anticorps HARS, anticorps Hars

Sujet: Histidyl-tRNA synthetase (HisRS, Jo-1) is an aminoacyl-tRNA synthetase that charges transfer RNAs (tRNAs) with a cognate histidine residue to produce histidyl-tRNA. Histidyl-tRNA is essential for the incorporation of histidine into proteins. Systemic autoimmune diseases lead to aminoacyl-tRNA synthetase autoantibodies that are directed against self structures. HisRS is an antigen in systemic autoimmune diseases such as rheumatic arthritis or polymyositis with associated interstitial lung disease, thrombocytopenia and Raynaud's phenomenon (vasospastic attacks that cause blood vessels to constrict). HisRS autoantibodies are unlike anti-synthetase antibodies because they bind the nonlinear, exposed epitopes on native HisRS when the enzyme is complexed to tRNA(His).Synonyms: HRS, HisRS, Histidine-tRNA ligase, JO1, Jo-1 antigen

Poids moléculaire: approx. 60 kDa

ID gène: 3035

NCBI Accession: NP_002100

UniProt: P12081