GTF3C2 anticorps

N° du produit ABIN272244

Détail du produit anti-GTF3C2 anticorps

Antigène: GTF3C2 (General Transcription Factor IIIC, Polypeptide 2, beta 110kDa (GTF3C2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GTF3C2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: This antibody detects endogenous levels of TFIIIC110 protein. (region surrounding Arg95)

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE!

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur GTF3C2

Antigène: GTF3C2 (General Transcription Factor IIIC, Polypeptide 2, beta 110kDa (GTF3C2))

Autre désignation: GTF3C2 / TFIIIC110 (GTF3C2 Produits)

Synonymes: anticorps TFIIIC110, anticorps GTF3C2, anticorps MGC155253, anticorps TF3C-beta, anticorps DKFZp469M0113, anticorps 1300004C11Rik, anticorps 2610510G03Rik, anticorps AI225816, anticorps AU041069, anticorps mKIAA0011, anticorps TFIIIC-BETA, anticorps general transcription factor IIIC subunit 2, anticorps general transcription factor IIIC, polypeptide 2, beta, anticorps Gtf3c2, anticorps GTF3C2

Sujet: RNA polymerase (pol) III synthesizes tRNA, 5s rRNA, 7SL RNA and U6 snRNA and is overexpressed in many transformed cell lines and tumors in vivo, since cells must duplicate its protein components before division. Therefore, in order to maintain rapid growth, cells must produce a high level of Pol III transcribed RNA, which requires the presence of the TFIIIB and TFIIIC2 transcription factor complexes. The TFIIIC2 complex is composed of five subunits, TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, that are overexpressed in adenovirus transformed cells as well as in malignant cells in vivo, such as ovarian carcinomas. TFIIIC2 recruits RNA pol III and TFIIIB to promoter elements and may be a key component in the deregulation of malignant cells. The TFIIIB complex includes the TATA-binding protein (TBP), TFIIB-related factor 1 (BRF1) and TFIIIB", the expression of which are also upregulated in transformed cells. In many carcinomas, the tumor suppressors retinoblastoma (RB) and p53 are inactivated, which affects their ability to bind and inactivate the function of TFIIIB.Synonyms: General transcription factor 3C polypeptide 2, KIAA0011, TF3C-beta, Transcription factor IIIC 110 kDa subunit, Transcription factor IIIC subunit beta

Poids moléculaire: approx. 100 kDa

ID gène: 2976

NCBI Accession: NP_001030598

UniProt: Q8WUA4