RFC5 anticorps (N-Term)

N° du produit ABIN2773876

Détail du produit anti-RFC5 anticorps

Antigène: RFC5 (Replication Factor C (Activator 1) 5, 36.5kDa (RFC5))

Épitope: N-Term

Reactivité: Humain, Souris, Rat, Chien, Cobaye, Cheval, Boeuf (Vache), Saccharomyces cerevisiae, Poisson zèbre (Danio rerio)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RFC5 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Séquence: METSALKQQE QPAATKIRNL PWVEKYRPQT LNDLISHQDI LSTIQKFINE

Homologie: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rat: 100%, Yeast: 90%, Zebrafish: 93%

Attributs du produit: This is a rabbit polyclonal antibody against RFC5. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human RFC5

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 340 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Détails sur RFC5

Antigène: RFC5 (Replication Factor C (Activator 1) 5, 36.5kDa (RFC5))

Autre désignation: RFC5 (RFC5 Produits)

Synonymes: anticorps zgc:110313, anticorps 2610020K06Rik, anticorps 2610209F07Rik, anticorps 36.5kDa, anticorps 36kDa, anticorps Recc5, anticorps RFC36, anticorps replication factor C (activator 1) 5, anticorps replication factor C subunit 5, anticorps replication factor C subunit 5 L homeolog, anticorps rfc5, anticorps Rfc5, anticorps RFC5, anticorps rfc5.L

Sujet: The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. RFC5 is the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system.The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
Alias Symbols: MGC1155, RFC36
Protein Interaction Partner: NAB2, XIAP, TRIM38, HUWE1, RFC4, SUMO2, PCNA, UBC, RPA3, RPA2, RPA1, EED, FBXO6, MMS19, VCP, ECT2, MDC1, MTMR2, RFC3, RFC2, RFC1, CAND1, CUL3, NEDD8, HDGF, SMARCAD1, PIDD1, MYC, RPAP3, MEPCE, RUVBL2, LRIF1, COPS6, MED31, RBM48, UNC119, EEF1A1, RAD17, BRD4
Protein Size: 340

Poids moléculaire: 38 kDa

ID gène: 5985

NCBI Accession: NM_007370, NP_031396

UniProt: P40937

Pathways: Telomere Maintenance, Réparation de l'ADN, DNA Replication, Synthesis of DNA