NEDD4-2 anticorps (Middle Region)

N° du produit ABIN2774731

Détail du produit anti-NEDD4-2 anticorps

Antigène: NEDD4-2 (NEDD4L) (E3 ubiquitin-protein ligase NEDD4-like (NEDD4L))

Épitope: Middle Region

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Cheval, Chien, Cobaye, Lapin, Poisson zèbre (Danio rerio)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NEDD4-2 est non-conjugé

Application: Western Blotting (WB)

Séquence: TVTLSAPLEG AKDSPVRRAV KDTLSNPQSP QPSPYNSPKP QHKVTQSFLP

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Attributs du produit: This is a rabbit polyclonal antibody against NEDD4L. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the middle region of human NEDD4L

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 955 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-NEDD4-2 (ABIN2774731)

Raikwar, Thomas: "Nedd4-2 isoforms ubiquitinate individual epithelial sodium channel subunits and reduce surface expression and function of the epithelial sodium channel." dans: American journal of physiology. Renal physiology, Vol. 294, Issue 5, pp. F1157-65, (2008) (PubMed).

Détails sur NEDD4-2

Antigène: NEDD4-2 (NEDD4L) (E3 ubiquitin-protein ligase NEDD4-like (NEDD4L))

Autre désignation: NEDD4L (NEDD4L Produits)

Synonymes: anticorps NEDD4-2, anticorps NEDD4.2, anticorps RSP5, anticorps hNEDD4-2, anticorps nedd4, anticorps nedd4-2, anticorps NEDD4, anticorps Nedd4-2, anticorps 1300012C07Rik, anticorps Nedd4b, anticorps neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase, anticorps NEDD4L, anticorps neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase S homeolog, anticorps neural precursor cell expressed, developmentally down-regulated gene 4-like, anticorps NEDD4L, anticorps LOC776799, anticorps nedd4l.S, anticorps Nedd4l

Sujet: NEDD4L is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. NEDD4L inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. NEDD4L promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. NEDD4L also promotes ubiquitination and degradation of SGK.
Alias Symbols: FLJ33870, KIAA0439, RSP5, hNedd4-2, NEDD4-2, NEDD4.2, hNEDD4-2
Protein Interaction Partner: NEDD4L, DAZAP2, UBE2D1, PTCH1, WNK3, TNK2, SNCA, SLC9A3, SMAD6, KCNH2, HCN1, SMAD3, UBE2N, SNA3, NOTCH1, UBE2D3, UBC, SGK1, KCNQ1, SGK3, GORAB, MED28, FBXL15, vpr, RNF11, AATF, YWHAQ, LAPTM5, SCNN1B, SMAD2, ARRDC3, ARRDC4, UBE2L3, UBE2D2, SMAD7, UBE2K, UB
Protein Size: 955

Poids moléculaire: 110 kDa

ID gène: 23327

NCBI Accession: NM_015277, NP_056092

Pathways: Negative Regulation of Transporter Activity