CITED2 anticorps (N-Term)
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- Antigène Voir toutes CITED2 Anticorps
- CITED2 (Cbp/p300-Interacting Transactivator, with Glu/Asp-Rich Carboxy-terminal Domain, 2 (CITED2))
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Épitope
- N-Term
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Reactivité
- Humain, Souris, Rat, Boeuf (Vache), Chien, Cobaye, Mouton
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp CITED2 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC)
- Séquence
- HIHYGAGNMN ATSGIRHAMG PGTVNGGHPP SALAPAARFN NSQFMGPPVA
- Homologie
- Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Sheep: 100%
- Attributs du produit
- This is a rabbit polyclonal antibody against CITED2. It was validated on Western Blot using a cell lysate as a positive control.
- Purification
- Affinity Purified
- Immunogène
- The immunogen is a synthetic peptide directed towards the N terminal region of human CITED2
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- Indications d'application
- Optimal working dilutions should be determined experimentally by the investigator.
- Commentaires
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Antigen size: 270 AA
- Restrictions
- For Research Use only
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- by
- Dr. Pengpeng Bi Lab, Department of Genetics, Franklin College of Arts and Sciences, University of Georgia
- No.
- #104256
- Date
- 10.02.2020
- Antigène
- CITED2
- Numéro du lot
- QC11500
- Application validée
- Western Blotting
- Contrôle positif
HEK293 cells overexpressing murine Cited2 (NCBI protein ID: NP_034958, 269 amino acids) from a transiently transfected expression plasmid
- Contrôle négative
Transient transfection with empty vector
- Conclusion
Passed. ABIN2776451 specifically recognizes Cited2 in human HEK293 cell lysates.
- Anticorps primaire
- ABIN2776451
- Anticorps secondaire
- Goat anti-Rabbit IgG (H+L), Superclonal Recombinant Secondary Antibody, HRP (Invitrogen, A27036, lot 20310171)
- Full Protocol
- Grow human HEK293 cells (Cell Biolabs, RV-101) in DMEM medium(Sigma, D5796) supplemented with FBS (Gemini, A59G00J) and Pen Strep (Gibco, 15140-122, lot 1999384), at 37°C and 5% CO2 in 2mL medium on a 6 well plate to 80% confluency.
- Transfect cells with 2µg per well pMXs-Puro murine Cited2 expression plasmid using PEI (Polysciences, 23966-1) following the manufacturer´s instructions.
- Lyse 1x106 cells in 100µl per well cold RIPA (Sigma, R0278).
- Determine total protein content of the lysates using Pierce BCA Protein Assay Kit (Thermo Fisher, 23225).
- Mix 40 µg of total protein with in 10 µL 4x Laemmli Sample Buffer (Biorad, 1610747) and subsequently separate them on a denaturing 12% PAGE gel (Biorad, 1610156) in a Biorad electrophoresis chamber for 1h at 100V.
- Transfer proteins onto Immobilon-PSQ PVDF Membrane (Sigma, ISEQ00010, lot R8CA8256B) with a Western blotting system for 1h at 110V.
- Block the membrane with blocking buffer (5% non-fat milk in TBST) for 1h at RT.
- Incubation with primary rabbit anti-CITED2 antibody (antibodies-online, ABIN2776451, lot QC11500) diluted 1:200 in blocking buffer ON at 4°C.
- Wash membrane 3x for 15min with TBST.
- Incubation with Goat anti-Rabbit IgG (H+L), Superclonal Recombinant Secondary Antibody, HRP (Invitrogen, A27036, lot 20310171) diluted 1:2000 in blocking buffer for 1h at RT.
- Wash membrane 5x for 15min with TBST.
- Reveal protein bands using SuperSignal West Dura (Thermo Scientific, 34075, TG268239) on a UVP ChemStudio touch (Analytik Jena, 84997092802).
- Notes
The CITED2 antibody ABIN2776451 reveals a protein of the expected molecular weight of the antigen in lysates of human HEK293 cells expressing murine Cited2. The protein band is only visible in the positive but not the negative controls.
Validation #104256 (Western Blotting)Validation ImagesProtocole -
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -20 °C
- Stockage commentaire
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- Antigène
- CITED2 (Cbp/p300-Interacting Transactivator, with Glu/Asp-Rich Carboxy-terminal Domain, 2 (CITED2))
- Autre désignation
- CITED2 (CITED2 Produits)
- Synonymes
- anticorps mrg1, anticorps p35srj, anticorps zgc:103418, anticorps CITED2, anticorps cite2, anticorps ASD8, anticorps MRG-1, anticorps MRG1, anticorps P35SRJ, anticorps VSD2, anticorps AI835299, anticorps ER154-like, anticorps Mrg1, anticorps Msg2, anticorps Cbp/p300-interacting transactivator, with Glu/Asp rich carboxy-terminal domain, 2, anticorps Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2, anticorps Cbp/p300 interacting transactivator with Glu/Asp rich carboxy-terminal domain 2, anticorps Cbp/p300-interacting transactivator 2, anticorps Cbp/p300-interacting transactivator, with Glu/Asp rich carboxy-terminal domain, 2 L homeolog, anticorps cited2, anticorps CITED2, anticorps cite2, anticorps Cited2, anticorps cited2.L
- Sujet
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CITED2 belongs to the CITED family. It interferes with the binding of transcription factors HIF-1a and STAT2 to p300/CBP.
Alias Symbols: MRG1, P35SRJ, ASD8, VSD2
Protein Interaction Partner: UBC, ELAVL1, EP300, SMAD3, SMAD2, LHX2, HIF1A, TFAP2C, LHX3, CREBBP, TFAP2A, TBP, TFAP2B,
Protein Size: 270 - Poids moléculaire
- 28 kDa
- ID gène
- 10370
- NCBI Accession
- NM_006079, NP_006070
- UniProt
- Q99967
- Pathways
- Tube Formation
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