SRSF1 anticorps (serine/arginine-Rich Splicing Factor 1) (C-Term)

Details for Product anti-SRSF1 Antibody No. ABIN2779013, Fournisseur: Connectez-vous pour afficher
Antigène
  • ASF
  • SF2
  • SF2p33
  • SFRS1
  • SRp30a
  • 1110054N12Rik
  • 5730507C05Rik
  • 6330415C05Rik
  • AI482334
  • AW491331
  • Asf
  • Sf2
  • Sfrs1
  • DKFZp469K2419
  • sfrs1
  • sfrs1b
  • wu:fb80g05
  • zgc:111894
  • zgc:65898
  • zgc:76897
  • asf
  • sf2
  • sf2p33
  • srp30a
  • serine and arginine rich splicing factor 1
  • serine/arginine-rich splicing factor 1
  • serine/arginine-rich splicing factor 1b
  • serine/arginine-rich splicing factor 1 L homeolog
  • SRSF1
  • Srsf1
  • srsf1b
  • srsf1.L
Épitope
C-Term
12
10
5
4
2
2
2
2
2
2
1
1
1
1
1
Reactivité
Boeuf (Vache), Chien, Cobaye, Cheval, Humain, Souris, Rat (Rattus), Poisson zèbre (Danio rerio)
69
29
28
8
8
4
4
3
3
3
3
3
3
2
2
1
1
1
Hôte
Lapin
63
6
Clonalité
Polyclonal
Conjugué
Cet anticorp SRSF1 est non-conjugé
2
2
2
2
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
65
26
26
13
11
8
5
3
2
1
1
Options
Fournisseur
Connectez-vous pour afficher
N° du produit (Fournisseur)
Connectez-vous pour afficher
Immunogène The immunogen is a synthetic peptide directed towards the C terminal region of human SFRS1
Blocking Peptide Blocking peptide for this product available: ABIN983639
Séquence EFVRKEDMTY AVRKLDNTKF RSHEGETAYI RVKVDGPRSP SYGRSRSRSR
Homologie Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 100%
Attributs du produit This is a rabbit polyclonal antibody against SFRS1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Plasmids, Primers & others Plasmids, Primers & others SRSF1 products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation SFRS1 (SRSF1 Antibody Extrait)
Sujet SFRS1 is a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. Multiple transcript variants encoding different isoforms have been found for this gene.Alternative mRNA splicing plays an important role in development and differentiation, many transcripts are spliced differently in distinct cell types and tissues. Both constitutive and alternative splicing occurs on spliceosomes, which are complex particles composed of small nuclear ribonucleoproteins (snRNPs) and non-snRNP proteins. The SR family of non-snRNP splicing factors is characterized by the presence of an RNA recognition motif and a serine- and arginine-rich (SR) domain. SR proteins are required at early stages of spliceosome assembly, have distinct but overlapping specificities for different pre-mRNAs, and can alter splice site choice, suggesting that they may be involved in the regulation of alternative splicing in vivo. Two of the SR proteins, ASF/SF2 (SFRS1) and SC35 (SFRS2, MIM 600813), have been extensively characterized.Alternative mRNA splicing plays an important role in development and differentiation, many transcripts are spliced differently in distinct cell types and tissues. Both constitutive and alternative splicing occurs on spliceosomes, which are complex particles composed of small nuclear ribonucleoproteins (snRNPs) and non-snRNP proteins. The SR family of non-snRNP splicing factors is characterized by the presence of an RNA recognition motif and a serine- and arginine-rich (SR) domain. SR proteins are required at early stages of spliceosome assembly, have distinct but overlapping specificities for different pre-mRNAs, and can alter splice site choice, suggesting that they may be involved in the regulation of alternative splicing in vivo. Two of the SR proteins, ASF/SF2 (SFRS1) and SC35 (SFRS2, MIM 600813), have been extensively characterized (Bermingham et al., 1995).[supplied by OMIM].
Alias Symbols: ASF, SF2, SFRS1, SF2p33, SRp30a, SRSF1
Protein Interaction Partner: TRAF3IP2, HUWE1, SPRTN, STAU1, UBC, MDM2, ZBTB1, RNF2, EZH2, EED, SUZ12, PARK2, SRPK2, SRPK1, SRPK3, FBXO6, YWHAE, U2AF2, PAN2, MPG, CIR1, FN1, CDK6, HNRNPA0, RNPS1, SRSF10, NXF1, PRPF4, BCL10, U2AF1, SRSF4, MAGOH, SMURF1, EIF4A3, ESR1, ECT2, BRCA1, BARD1
Protein Size: 248
Poids moléculaire 27 kDa
ID gène 6426
NCBI Accession NM_006924, NP_008855
UniProt Q07955
Pathways Ribonucleoprotein Complex Subunit Organization
Indications d'application Optimal working dilutions should be determined experimentally by the investigator.
Commentaires

Antigen size: 248 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Avoid repeated freeze-thaw cycles.
Stock -20 °C
Stockage commentaire For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Images (Fournisseur)
Western Blotting (WB) image for anti-serine/arginine-Rich Splicing Factor 1 (SRSF1) (C-Term) antibody (ABIN2779013) WB Suggested Anti-SFRS1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Posi...
Western Blotting (WB) image for anti-serine/arginine-Rich Splicing Factor 1 (SRSF1) (C-Term) antibody (ABIN2779013) WB Suggested Anti-SFRS1 Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:.2500 Positi...
Immunohistochemistry (IHC) image for anti-serine/arginine-Rich Splicing Factor 1 (SRSF1) (C-Term) antibody (ABIN2779013) Rabbit Anti-SRSF1 Antibody Formalin Fixed Paraffin Embedded Tissue: Human heart Tis...
Produit citée dans: Li, Xia, Huang, Chen, Su, Li, Wang, Ding, Shao: "A strategy to rapidly identify the functional targets of microRNAs by combining bioinformatics and mRNA cytoplasmic/nucleic ratios in culture cells." dans: FEBS letters, Vol. 584, Issue 14, pp. 3198-202, 2010 (PubMed).

Background publications Velazquez-Dones, Hagopian, Ma, Zhong, Zhou, Ghosh, Fu, Adams: "Mass spectrometric and kinetic analysis of ASF/SF2 phosphorylation by SRPK1 and Clk/Sty." dans: The Journal of biological chemistry, Vol. 280, Issue 50, pp. 41761-8, 2005 (PubMed).

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