GBL anticorps (Middle Region)

N° du produit ABIN2787309

Détail du produit anti-GBL anticorps

Antigène: GBL (G protein beta subunit-like (GBL))

Épitope: Middle Region

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Chévre, Cobaye, Cheval, Lapin, Poisson zèbre (Danio rerio)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GBL est non-conjugé

Application: Western Blotting (WB)

Séquence: CAFSGDSQYI VTASSDNLAR LWCVETGEIK REYGGHQKAV VCLAFNDSVL

Homologie: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Attributs du produit: This is a rabbit polyclonal antibody against GBL. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the middle region of human GBL

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 326 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Détails sur GBL

Antigène: GBL (G protein beta subunit-like (GBL))

Autre désignation: GBL (GBL Produits)

Sujet: GBL is the subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
Alias Symbols: MGC111011, GBL, LST8, POP3, WAT1, GbetaL
Protein Interaction Partner: MTOR, EIF4EBP1, RPTOR, AKT1S1, PRR5L, FKBP8, SUZ12, PRMT1, STAT1, RICTOR, UBC, ATXN1, A2M, DEPTOR, ULK1, RPS6KB1, SQSTM1, Cul4a, Dtl, PRR5, CCT2, KRT85, RHEB,
Protein Size: 326

Poids moléculaire: 36 kDa

ID gène: 64223

NCBI Accession: NM_022372, NP_071767

UniProt: Q9BVC4

Pathways: Signalisation PI3K-Akt, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Autophagy, CXCR4-mediated Signaling Events, BCR Signaling, L'effet Warburg