ARPC2 anticorps

N° du produit ABIN2855249

Détail du produit anti-ARPC2 anticorps

Antigène: ARPC2 (Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ARPC2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Réactivité croisée: Humain, Souris, Rat, Poisson zèbre (Danio rerio)

Attributs du produit: Rabbit Polyclonal antibody to ARPC2 (actin related protein 2/3 complex, subunit 2, 34 kDa)
ARPC2 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the center region of human ARPC2. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:5000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: rat kidney , mouse kidney

Restrictions: For Research Use only

Independent Validation (mIHC)

Validation #104342 (Multiplex Immunohistochemistry)

by: Akoya Biosciences

No.: #104342

Date: 20.04.2021

Antigène: ARPC2

Numéro du lot: 39911

Application validée: Multiplex Immunohistochemistry

Contrôle positif:

FFPE normal human cortex

Contrôle négative:

Unlabeled control (FFPE normal human cortex)

Conclusion:

Passed. The anti-ARPC2 antibody ABIN2855249 produced staining in neuronal processes throughout the human cortex.

Validation Images

FFPE normal human cortex tissue section labeled with anti-ARPC2 antibody ABIN2855249 (cyan; conjugated to fluorophore AF488) after EDTA antigen retrieval. MAP2, neurofilament, and GFAP were labeled with anti-MAP2 antibody ABIN125739 (green; conjugated to fluorophore ATTO 550), anti-neurofilament antibody (red; conjugated to fluorophore AF488), and anti-GFAP antibody (purple; conjugated to fluorophore AF647) respectively.

FFPE normal human cortex tissue section labeled with anti-ARPC2 antibody ABIN2855249 (cyan; conjugated to fluorophore AF488) after EDTA antigen retrieval. GFAP was labeled with anti-ARPC2 antibody (red; conjugated to fluorophore AF647).

Protocole

Anticorps primaire: ABIN2855249

Full Protocol:

• Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
• Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual fresh-frozen tissue protocol.
• Conjugation of the anti-ARPC2 antibody ABIN2855249 to an oligo barcode used to bind oligo-conjugated fluorophore AF488.

Notes:

• No signal was detected in unlabeled specimens.

• Specific staining of ARPC2 was observed with human FFPE cortical tissue sections with tris-EDTA antigen retrieval.

• Anti-ARPC antibody ABIN2855249 was weakly compatible with staining of fresh frozen mouse tissue

• Optimal staining and signal to noise ratios were obtained if tissue was pre-treated for autofluorescence removal (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur ARPC2

Antigène: ARPC2 (Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2))

Autre désignation: actin related protein 2/3 complex subunit 2 (ARPC2 Produits)

Synonymes: anticorps arc34, anticorps p34-arc, anticorps pnas-139, anticorps pro2446, anticorps Arpc2, anticorps Arc-p34, anticorps 2210023N03Rik, anticorps 34kDa, anticorps p34-Arc, anticorps fk84a07, anticorps wu:fa22f07, anticorps wu:fk84a07, anticorps zgc:77769, anticorps ARC34, anticorps PNAS-139, anticorps actin related protein 2/3 complex subunit 2, anticorps actin related protein 2/3 complex, subunit 2, 34kDa, anticorps actin related protein 2/3 complex, subunit 2, anticorps actin related protein 2/3 complex subunit 2 S homeolog, anticorps ARPC2, anticorps arpc2, anticorps Arpc2, anticorps arpc2.S

Sujet: This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined.

Cellular Localization: Cytoplasm , cytoskeleton , Cell projection

Poids moléculaire: 34 kDa

ID gène: 10109

UniProt: O15144

Pathways: Signalisation RTK, Regulation of Actin Filament Polymerization