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Calretinin anticorps

CALB2 Reactivité: Humain WB, IHC (p), IF, ICC, IHC (fro) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN2855596
  • Antigène Voir toutes Calretinin (CALB2) Anticorps
    Calretinin (CALB2) (Calbindin 2 (CALB2))
    Reactivité
    • 83
    • 56
    • 56
    • 8
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Humain
    Hôte
    • 62
    • 20
    • 8
    • 1
    Lapin
    Clonalité
    • 57
    • 33
    Polyclonal
    Conjugué
    • 62
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Calretinin est non-conjugé
    Application
    • 71
    • 36
    • 30
    • 16
    • 16
    • 13
    • 13
    • 13
    • 10
    • 7
    • 7
    • 7
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
     Réactivité croisée
    Humain, Souris, Rat
    Attributs du produit
    Rabbit Polyclonal antibody to Calretinin (calbindin 2)
    Calretinin antibody
    Purification
    Purified by antigen-affinity chromatography.
    Immunogène
    Recombinant protein encompassing a sequence within the center region of human Calretinin. The exact sequence is proprietary.
    Isotype
    IgG
  • Indications d'application
    WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
    Commentaires

    Positive Control: A431 , Mouse brain , human CALB2-transfected 293T cells , rat brain

    Validation: Overexpression

    Restrictions
    For Research Use only
  • Validation #103867 (Immunohistochemistry)
    'Independent Validation' signe
    by
    Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin
    No.
    #103867
    Date
    29.05.2019
    Antigène
    Calretinin
    Numéro du lot
    39876
    Application validée
    Immunohistochemistry
    Contrôle positif

    Adult (24 months) mouse and dog brain

    Contrôle négative

    We incubated slices overnight with the blocking solution only and then processed them with the secondary antibody.

    Conclusion

    ABIN2855596 works in IHC-P with microwave antigen retrieval pretreatment at an optimal concentration of 1:200.

    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    ABIN2855596
    Anticorps secondaire
    goat anti-rabbit AF488-conjugated antibody (Invitrogen by Thermo Fisher Scientific, A11034, lot 1971418)
    Full Protocol
    • Obtain dog samples after autopsy and immersion-fix in buffered formaldehyde for 16h.
    • Perfuse mice with paraformaldehyde 4% in 0.1M phosphate buffer pH 7.4 and post-fix in the same fixative for an additional 2h at RT.
    • Wash, dehydrate, and embed samples in paraffin wax.
    • Cut with a microtome into 6µm sections and mount on glass slides.
    • After paraffin removal, process sections by microwave antigen retrieval for 10min (95-100°C) in 10mM sodium citrate buffer (pH 6.0).
    • Let sections cool for 10min.
    • Wash sections under running water for 5-10min.
    • Wash sections 2x 5min with distilled water.
    • Wash sections 5min with 0.01M PBS.
    • Incubate sections in PBS containing 1% albumin from chicken egg white (Sigma, A5378) for 1h at RT to block non-specific binding sites.
    • Incubate sections with primary rabbit anti-Calretinin antibody (antibodies-online, ABIN2855596, lot 39876) diluted 1:200 in PBS-BSA-PLL at 4°C.
    • Wash sections 3x 5min with 0.01M PBS.
    • Incubate sections with secondary goat anti-rabbit AF488-conjugated antibody (Invitrogen by Thermo Fisher Scientific, A11034, lot 1971418) diluted 1:500 in 0.1M PBS for 1h at RT.
    • Wash sections 3x 5min with 0.01M PBS.
    • Mount specimens in Fluoroshield (Sigma, F6182, lot MKCB0153V).
    • Acquire images with a fluorescence microscope and appropriate filter settings for AF488, e.g. Leica DM 6000B fluorescence microscope equipped with the manufacturer’s FITC filter set and digital camera at magnifications of 20x, 40x, and 63x. Parameters for image acquisition (Exposure 2.2s, Gain 2.3x, Saturation 1.5, Gamma 1.10) unchanged for all images.
    Notes

    For the incubation with the primary antibody ABIN2669973 1:100, 1:500, and 1:1000 dilutions were also tested.

  • Format
    Liquid
    Concentration
    0.75 mg/mL
    Buffer
    0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal
    Agent conservateur
    Thimerosal (Merthiolate)
    Précaution d'utilisation
    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Bradshaw, Trossbach, Köber, Walter, Prikulis, Weggen, Korth: "Disrupted in Schizophrenia 1 regulates the processing of reelin in the perinatal cortex." dans: Schizophrenia research, Vol. 215, pp. 506-513, (2021) (PubMed).

  • Antigène
    Calretinin (CALB2) (Calbindin 2 (CALB2))
    Autre désignation
    calbindin 2 (CALB2 Produits)
    Synonymes
    anticorps CAB29, anticorps CAL2, anticorps CR, anticorps calb2l, anticorps wu:fq18e08, anticorps zgc:73115, anticorps calb2, anticorps wu:fq17g09, anticorps zgc:73099, anticorps calbindin 2, anticorps calbindin 2a, anticorps calbindin 2b, anticorps CALB2, anticorps Calb2, anticorps calb2a, anticorps calb2b
    Sujet
    This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. Three alternatively spliced transcript variants that encode different proteins have been described.

    Poids moléculaire
    32 kDa
    ID gène
    794
    UniProt
    P22676
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