PSMD14 anticorps (Center)

N° du produit ABIN2856031

Détail du produit anti-PSMD14 anticorps

Antigène: PSMD14 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 14 (PSMD14))

Épitope: Center

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PSMD14 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Attributs du produit: Rabbit Polyclonal antibody to MAD1 (MAD1 mitotic arrest deficient-like 1 (yeast))
MAD1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the center region of human MAD1. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

Commentaires:

Positive Control: 293T , A431 , HeLa , HepG2 , A375

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Références pour anticorps anti-PSMD14 (ABIN2856031)

Alfonso-Pérez, Hayward, Holder, Gruneberg, Barr: "MAD1-dependent recruitment of CDK1-CCNB1 to kinetochores promotes spindle checkpoint signaling." dans: The Journal of cell biology, Vol. 218, Issue 4, pp. 1108-1117, (2020) (PubMed).

Détails sur PSMD14

Antigène: PSMD14 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 14 (PSMD14))

Autre désignation: MAD1 (PSMD14 Produits)

Synonymes: anticorps PAD1, anticorps POH1, anticorps RPN11, anticorps 2610312C03Rik, anticorps 3200001M20Rik, anticorps AA986732, anticorps Pad1, anticorps Poh1, anticorps rpm11, anticorps zgc:162272, anticorps proteasome 26S subunit, non-ATPase 14, anticorps proteasome (prosome, macropain) 26S subunit, non-ATPase, 14, anticorps 26S proteasome non-ATPase regulatory subunit 14, anticorps proteasome 26S subunit, non-ATPase 14 L homeolog, anticorps 26S proteasome-associated pad1 homolog, anticorps PSMD14, anticorps Psmd14, anticorps rpn-11, anticorps psmd14, anticorps psmd14.L

Sujet: MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Three transcript variants encoding the same protein have been found for this gene.

Cellular Localization: Nucleus , Kinetochore

Poids moléculaire: 83 kDa

ID gène: 8379

Pathways: Mitotic G1-G1/S Phases, DNA Replication, M Phase, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Ubiquitin Proteasome Pathway