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TJP1 anticorps (N-Term)

Name: TJP1 anticorps (N-Term)
Brand: antibodies-online
SKU: ABIN2856624
Price: 660.63 EUR
Availability: InStock

KO Validated TJP1 Reactivité: Humain WB, IP, IF, ICC Hôte: Lapin Polyclonal unconjugated

WB Image Sample(30 ug whole cell lysate) A:H1299 5% SDS PAGE antibody diluted at 1:3000

IHC-P Image Immunohistochemical analysis of paraffin-embedded H661 xenograft, using ZO-1, antibody at 1:250 dilution.

IHC

WB Image Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N1N2], N-term , diluted at 1:500.

IHC-P Image ZO-1 antibody [N1N2], N-term detects ZO-1 protein at cell membrane and cytoplasm in mouse intestine by immunohistochemical analysis.

IHC

ICC/IF Image ZO-1 antibody [N1N2], N-term detects TJP1 protein at junction by confocal immunofluorescent analysis.

IHC-P Image ZO-1 antibody [N1N2], N-term detects ZO-1 protein at cell membrane and cytoplasm in mouse testis by immunohistochemical analysis.

IHC

ICC/IF Image ZO-1 antibody [N1N2], N-term detects ZO-1 protein at cell membrane by immunofluorescent analysis.

Identification of SLC26A3 as a CFTR and ZO-1 binding partner and effects on their interaction.

8 images

(1 reference)

N° du produit ABIN2856624

Fiche de données

Antigène Voir toutes TJP1 Anticorps

TJP1 (Tight Junction Protein 1 (TJP1))
Épitope
16

6

4

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term
Reactivité
54

32

30

20

18

9

6

5

4

4

2

2

1

1

1

1

1

1

1

1
Humain
Hôte
48

4

3

1

1
Lapin
Anticorps secondaires appropriés
Clonalité
50

7
Polyclonal
Conjugué
39

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
Cet anticorp TJP1 est non-conjugé
Application
52

20

18

15

14

13

12

12

10

8

6

3

2

1
Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Réactivité croisée

Humain, Souris

Attributs du produit

Rabbit Polyclonal antibody to ZO-1 (tight junction protein 1 (zona occludens 1))
ZO-1 antibody [N1N2], N-term

Purification

Purified by antigen-affinity chromatography.

Classe de qualité

KO Validated

Immunogène

Recombinant protein encompassing a sequence within the N-terminus region of human ZO-1. The exact sequence is proprietary.

Isotype

IgG

Top Product

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anti-Tight Junction Protein 1 (TJP1) (AA 1551-1702) antibody
TJP1 Reactivité: Humain, Souris, Rat, Porc, Boeuf (Vache) WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) Hôte: Lapin Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) (C-Term) antibody
TJP1 Reactivité: Humain, Souris, Rat, Porc, Boeuf (Vache), Chien, Cobaye, Lapin, Cheval, Singe, Poulet, Avian, Cat, Âne, Chévre, Hamster, Mouton WB, IF, IHC (p), IHC (fro) Hôte: Chèvre Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) (AA 1600-1700) antibody
TJP1 Reactivité: Humain WB, IHC, IF Hôte: Lapin Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) (Internal Region) antibody
Verified TJP1 Reactivité: Humain ELISA, IF Hôte: Chèvre Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) antibody
TJP1 Reactivité: Humain WB, IHC, ELISA, FACS, FM Hôte: Lapin Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) antibody
TJP1 Reactivité: Humain, Souris, Rat, Porc WB, IHC, ELISA, IF, ICC Hôte: Lapin Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) (AA 1048-1247) antibody
TJP1 Reactivité: Humain WB, IF Hôte: Souris Monoclonal 1-ZO unconjugated

anti-Tight Junction Protein 1 (TJP1) (AA 1178-1527) antibody
TJP1 Reactivité: Humain, Souris, Rat WB, IHC (p) Hôte: Lapin Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) (AA 1570-1600), (C-Term) antibody
TJP1 Reactivité: Humain WB, FACS, IHC (p) Hôte: Lapin Polyclonal RB15419 unconjugated

anti-Tight Junction Protein 1 (TJP1) antibody
TJP1 Reactivité: Souris WB, IHC, IP Hôte: Rat Monoclonal unconjugated

Indications d'application

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires

Positive Control: HeLa
Validation: KO/KD

Restrictions

For Research Use only
Format

Liquid

Concentration

0.22 mg/mL

Buffer

1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Agent conservateur

ProClin

Précaution d'utilisation

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Zhang, Heruth, Wu, Zhang, Nsumu, Shortt, Li, Jiang, Wang, Friesen, Li, Ye: "Functional characterization of SLC26A3 c.392C>G (p.P131R) mutation in intestinal barrier function using CRISPR/CAS9-created cell models." dans: Cell & bioscience, Vol. 9, pp. 40, (2019) (PubMed).
Antigène

TJP1 (Tight Junction Protein 1 (TJP1))

Autre désignation

tight junction protein 1 (TJP1 Produits)

Synonymes

anticorps ZO-1, anticorps ZO1, anticorps zo1, anticorps tight junction protein 1, anticorps tight junction protein ZO-1, anticorps TJP1, anticorps Tjp1, anticorps LOC396567

Sujet

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene.

Cellular Localization: Cell membrane, Peripheral membrane protein, Cytoplasmic side , Cell junction , tight junction

Poids moléculaire

195 kDa

ID gène

7082

UniProt

Q07157

Pathways

Carbohydrate Homeostasis, Cell-Cell Junction Organization