GAPDH anticorps

N° du produit ABIN2857072

Détail du produit anti-GAPDH anticorps

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GAPDH est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Réactivité croisée: Bactéries, C. elegans, Chanos chanos, Poulet, Boeuf (Vache), Chien, Drosophila melanogaster, E. coli, Poisson, Hamster, Humain, Insectes, Singe, Moustique, Souris, Nematode, Ochotona princeps, Porc, Plantes, Lapin, Rat, Saccharomyces cerevisiae, Poisson zèbre (Danio rerio)

Attributs du produit: Rabbit Polyclonal antibody to GAPDH (glyceraldehyde-3-phosphate dehydrogenase)
GAPDH antibody

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:5000-1:100000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: PC-12 , Rat2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , E.coli M15

Validation: Orthogonal

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.11 mg/mL

Buffer: 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7), 0.025 % ProClin 300

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Références pour anticorps anti-GAPDH (ABIN2857072)

Zhang, Dai, Zhao, Wang, Dou, Zhuang, Chen, Zhao et al.: "MiR-874-3p represses the migration and invasion yet promotes the apoptosis and cisplatin sensitivity via being sponged by long intergenic non-coding RNA 00922 (LINC00922) and targeting ..." dans: Bioengineered, Vol. 13, Issue 3, pp. 7082-7104, (2022) (PubMed).

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Liao, Zhou, Zhang: "Effects of miR‑195‑5p on cell proliferation and apoptosis in gestational diabetes mellitus via targeting EZH2." dans: Molecular medicine reports, Vol. 22, Issue 2, pp. 803-809, (2020) (PubMed).

Xu, Li, Wang, Wu, Wang, Chen, Deng, Zhang, Wu, Wan, Liu, Huang, Hu, Zeng, Zhou: "Mutated SASH1 promotes Mitf expression in a heterozygous mutated SASH1 knock‑in mouse model." dans: International journal of molecular medicine, Vol. 46, Issue 3, pp. 1118-1134, (2020) (PubMed).

Liang, Chang, Sheu, Lin, Chung, Teng, Suk: "The Antihistamine Deptropine Induces Hepatoma Cell Death through Blocking Autophagosome-Lysosome Fusion." dans: Cancers, Vol. 12, Issue 6, (2020) (PubMed).

Liu, Du, Rao, Liu, Qu: "Long non-coding RNA NPBWR1-2 affects the development of ovarian cancer via multiple microRNAs." dans: Oncology letters, Vol. 20, Issue 1, pp. 685-692, (2020) (PubMed).

Chang, Kam, Chao, Zhao, Chen, Chung, Li, Wu, Lee: "Berberine Derivatives Suppress Cellular Proliferation and Tumorigenesis In Vitro in Human Non-Small-Cell Lung Cancer Cells." dans: International journal of molecular sciences, Vol. 21, Issue 12, (2020) (PubMed).

Low, Lee, Lee, Wang, Chen, Chien, Li, Chang: "Reciprocal Regulation Between Indoleamine 2,3-Dioxigenase 1 and Notch1 Involved in Radiation Response of Cervical Cancer Stem Cells." dans: Cancers, Vol. 12, Issue 6, (2020) (PubMed).

Möltgen, Piumatti, Massafra, Metzger, Jaehde, Kalayda: "Cisplatin Protein Binding Partners and Their Relevance for Platinum Drug Sensitivity." dans: Cells, Vol. 9, Issue 6, (2020) (PubMed).

Bahraoui, Serrero, Planès: "HIV-1 Tat - TLR4/MD2 interaction drives the expression of IDO-1 in monocytes derived dendritic cells through NF-κB dependent pathway." dans: Scientific reports, Vol. 10, Issue 1, pp. 8177, (2020) (PubMed).

Chen, Wu, Ho, Gung, Tsai, Orekhov, Sobenin, Lin, Yet: "Exposure to Zinc Oxide Nanoparticles Disrupts Endothelial Tight and Adherens Junctions and Induces Pulmonary Inflammatory Cell Infiltration." dans: International journal of molecular sciences, Vol. 21, Issue 10, (2020) (PubMed).

Baumann, Hägele, Mochayedi, Drebant, Vent, Blobner, Noll, Nickel, Schumacher, Boos, Daniel, Wendler, Volkmar, Strobel, Offringa: "Proimmunogenic impact of MEK inhibition synergizes with agonist anti-CD40 immunostimulatory antibodies in tumor therapy." dans: Nature communications, Vol. 11, Issue 1, pp. 2176, (2020) (PubMed).

Yang, Hsieh, Du, Yen, Hung: "Sequential Interferon β-Cisplatin Treatment Enhances the Surface Exposure of Calreticulin in Cancer Cells via an Interferon Regulatory Factor 1-Dependent Manner." dans: Biomolecules, Vol. 10, Issue 4, (2020) (PubMed).

Wang, Yang, Liu, Chun-Kit Tong, Zhu, Malampati, Gopalkrishnashetty Sreenivasmurthy, Cheung, Iyaswamy, Su, Lu, Song, Li: "A Curcumin Derivative Activates TFEB and Protects Against Parkinsonian Neurotoxicity inVitro." dans: International journal of molecular sciences, Vol. 21, Issue 4, (2020) (PubMed).

Liu, Chen, Lii, Jhuang, Huang, Li, Yao: "A Diterpenoid, 14-Deoxy-11, 12-Didehydroandrographolide, in Andrographis paniculata Reduces Steatohepatitis and Liver Injury in Mice Fed a High-Fat and High-Cholesterol Diet." dans: Nutrients, Vol. 12, Issue 2, (2020) (PubMed).

Loh, Tsai, Huang, Yu, Kuo, Chao, Chou, Tsai, Lee: "Effects of Andrographolide on Intracellular pH Regulation, Cellular Migration, and Apoptosis in Human Cervical Cancer Cells †." dans: Cancers, Vol. 12, Issue 2, (2020) (PubMed).

Yang, Zhu, Tong, Iyaswamy, Xie, Zhu, Sreenivasmurthy, Senthilkumar, Cheung, Song, Zhang, Li: "A stress response p38 MAP kinase inhibitor SB202190 promoted TFEB/TFE3-dependent autophagy and lysosomal biogenesis independent of p38." dans: Redox biology, Vol. 32, pp. 101445, (2020) (PubMed).

Chang, Chu, Tsai, Lai, Chen: "Dopamine D3 receptor and GSK3β signaling mediate deficits in novel object recognition memory within dopamine transporter knockdown mice." dans: Journal of biomedical science, Vol. 27, Issue 1, pp. 16, (2020) (PubMed).

Wang, Wang, Lin, Kao, Hung, Teng, Tsai, Chi: "Progerin in muscle leads to thermogenic and metabolic defects via impaired calcium homeostasis." dans: Aging cell, Vol. 19, Issue 2, pp. e13090, (2020) (PubMed).

Huang, Chyuan, Shiue, Yu, Hsu, Hsu: "Lovastatin-mediated MCF-7 cancer cell death involves LKB1-AMPK-p38MAPK-p53-survivin signalling cascade." dans: Journal of cellular and molecular medicine, Vol. 24, Issue 2, pp. 1822-1836, (2020) (PubMed).

Détails sur GAPDH

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Autre désignation: glyceraldehyde-3-phosphate dehydrogenase (GAPDH Produits)

Synonymes: anticorps G3PD, anticorps GAPD, anticorps Gapd, anticorps BEST:GH12586, anticorps CG12055, anticorps Dmel\\CG12055, anticorps GA3PDH, anticorps GADPH, anticorps GAP, anticorps GAPDH, anticorps GAPDH I, anticorps GAPDH-1, anticorps GAPDH1, anticorps GAPDHI, anticorps Gapdh, anticorps Gapdh-1, anticorps Gapdh43E, anticorps gadph, anticorps gapdh, anticorps gapdh-1, anticorps gh12586, anticorps cb609, anticorps gapd, anticorps mg:bb02e05, anticorps wu:fb33a10, anticorps wu:ft80f05, anticorps KNC-NDS6, anticorps g3pd, anticorps G3PDH, anticorps glyceraldehyde-3-phosphate dehydrogenase, anticorps Glyceraldehyde 3 phosphate dehydrogenase 1, anticorps glyceraldehyde-3-phosphate dehydrogenase S homeolog, anticorps glyceraldehyde-3-phosphate dehydrogenase, type I, anticorps olfactory receptor 8K3, anticorps GAPDH, anticorps Gapdh, anticorps Gapdh1, anticorps gapdh, anticorps gapdh.S, anticorps gapDH, anticorps LOC100404960, anticorps LOC614985

Sujet: The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.

Cellular Localization: Cytoplasm , perinuclear region , Membrane

Poids moléculaire: 36 kDa

ID gène: 2597

UniProt: P04406