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HES4 anticorps (AA 94-221)

HES4 Reactivité: Humain WB, ELISA, ICC Hôte: Mouton Polyclonal unconjugated
N° du produit ABIN291868
  • Antigène Voir toutes HES4 Anticorps
    HES4 (Hairy and Enhancer of Split 4 (HES4))
    Épitope
    • 2
    • 2
    • 1
    • 1
    • 1
    AA 94-221
    Reactivité
    • 9
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 5
    • 2
    • 1
    • 1
    Mouton
    Clonalité
    • 9
    Polyclonal
    Conjugué
    • 8
    • 1
    Cet anticorp HES4 est non-conjugé
    Application
    • 8
    • 4
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
    Specificité
    Selected for its ability to recognize human HES-4 in the applications listed below. In direct ELISAs and Western Blots, this antibody shows less than 1 % cross-reactivity with rhHES-1, rhHES-2, rhHES-5, and rhHES-7.
    Purification
    Affinity purified
    Immunogène
    E. coli-derived rhHES-4 (aa94-221).
    Isotype
    IgG
    Top Product
    Discover our top product HES4 Anticorps primaire
  • Indications d'application
    Approved: ELISA, ICC, WB

    Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human HES-4. The detection limit for rhHES-4 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect HES-4 in HEK293 cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by fluorochrome-coupled anti-sheep IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human HES-4. The detection limit for rhHES-4 is approximately 0.2 ng/well.
    Commentaires

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    deionized water
    Concentration
    Lot specific
    Buffer
    Lyophilized.
  • Antigène
    HES4 (Hairy and Enhancer of Split 4 (HES4))
    Autre désignation
    HES4 (HES4 Produits)
    Synonymes
    anticorps bHLHb42, anticorps XHairy2a, anticorps Xhairy2, anticorps Xhairy2b, anticorps hairy2, anticorps hairy2a, anticorps hairy2b, anticorps hes1, anticorps hes4a, anticorps hes4b, anticorps Xhairy2a, anticorps hes family bHLH transcription factor 4, anticorps hes family bHLH transcription factor 4 L homeolog, anticorps hes family bHLH transcription factor 4 S homeolog, anticorps HES4, anticorps hes4, anticorps hes4.L, anticorps hes4.S
    Sujet
    Name/Gene ID: HES4

    Synonyms: HES4, BHLHb42, Transcription factor HES-4, BHLH factor Hes4, Hairy and enhancer of split 4, HHES4
    ID gène
    57801
    UniProt
    Q9HCC6
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